Abstract
Background: Novel cancer biomarkers like microRNA (miRNA) are promising tools to gain a better understanding of lung cancer pathology and yield important information to guide therapy. In recent years, new less invasive methods for the diagnosis and staging of NSCLC have become key tools in thoracic oncology and the worldwide spread of endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA). However, appropriate specimen handling is mandatory to achieve adequate results and reproducibility. The aim of this single centre prospective study was to evaluate the feasibility of a complete miRNA expression profile in fresh NSCLC cell lines obtained by EBUS-TBNA. Methods: Patients with proven NSCLC underwent EBUS-TBNA for the diagnosis of suspect lymph node metastasis, and cytological specimens were collected for epithelial cell culture and miRNA expression analysis. To validate the miRNA expression profile, we compared the results from EBUS-TBNA NSCLC specimens with those obtained from formalin-fixed paraffin-embedded (FFPE) mediastinoscopy specimens. Results: Analysis of the miRNA expression profiles of three independent EBUS-TBNA-derived primary cell lines allowed the screening of 377 different human miRNAs. One hundred and fifty miRNAs were detected in all cell lines. Analysis of the miRNA expression profile in mediastinoscopy specimens showed a strong similarity in the clusters analysed. Conclusions: The miRNA expression profile is feasible and reliable in EBUS-TBNA specimens. Validation of this protocol in fresh cytological specimens represents an effective and reproducible method to correlate translational and clinical research.
| Original language | English |
|---|---|
| Pages (from-to) | 408-415 |
| Number of pages | 8 |
| Journal | Journal of Thoracic Disease |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
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Keywords
- Bronchoscopy
- Endobronchial ultrasound
- Non-small cell lung cancer (NSCLC)
- Translational research
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
MicroRNA expression profile in primary lung cancer cells lines obtained by endobronchial ultrasound transbronchial needle aspiration. / Guarize, Juliana; Bianchi, Fabrizio; Marino, Elena; Belloni, Elena; Vecchi, Manuela; Donghi, Stefano; Lo Iacono, Giorgio; Casadio, Chiara; Cuttano, Roberto; Barberis, Massimo; Di Fiore, Pier Paolo; Petrella, Francesco; Spaggiari, Lorenzo.
In: Journal of Thoracic Disease, Vol. 10, No. 1, 01.01.2018, p. 408-415.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNA expression profile in primary lung cancer cells lines obtained by endobronchial ultrasound transbronchial needle aspiration
AU - Guarize, Juliana
AU - Bianchi, Fabrizio
AU - Marino, Elena
AU - Belloni, Elena
AU - Vecchi, Manuela
AU - Donghi, Stefano
AU - Lo Iacono, Giorgio
AU - Casadio, Chiara
AU - Cuttano, Roberto
AU - Barberis, Massimo
AU - Di Fiore, Pier Paolo
AU - Petrella, Francesco
AU - Spaggiari, Lorenzo
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Background: Novel cancer biomarkers like microRNA (miRNA) are promising tools to gain a better understanding of lung cancer pathology and yield important information to guide therapy. In recent years, new less invasive methods for the diagnosis and staging of NSCLC have become key tools in thoracic oncology and the worldwide spread of endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA). However, appropriate specimen handling is mandatory to achieve adequate results and reproducibility. The aim of this single centre prospective study was to evaluate the feasibility of a complete miRNA expression profile in fresh NSCLC cell lines obtained by EBUS-TBNA. Methods: Patients with proven NSCLC underwent EBUS-TBNA for the diagnosis of suspect lymph node metastasis, and cytological specimens were collected for epithelial cell culture and miRNA expression analysis. To validate the miRNA expression profile, we compared the results from EBUS-TBNA NSCLC specimens with those obtained from formalin-fixed paraffin-embedded (FFPE) mediastinoscopy specimens. Results: Analysis of the miRNA expression profiles of three independent EBUS-TBNA-derived primary cell lines allowed the screening of 377 different human miRNAs. One hundred and fifty miRNAs were detected in all cell lines. Analysis of the miRNA expression profile in mediastinoscopy specimens showed a strong similarity in the clusters analysed. Conclusions: The miRNA expression profile is feasible and reliable in EBUS-TBNA specimens. Validation of this protocol in fresh cytological specimens represents an effective and reproducible method to correlate translational and clinical research.
AB - Background: Novel cancer biomarkers like microRNA (miRNA) are promising tools to gain a better understanding of lung cancer pathology and yield important information to guide therapy. In recent years, new less invasive methods for the diagnosis and staging of NSCLC have become key tools in thoracic oncology and the worldwide spread of endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA). However, appropriate specimen handling is mandatory to achieve adequate results and reproducibility. The aim of this single centre prospective study was to evaluate the feasibility of a complete miRNA expression profile in fresh NSCLC cell lines obtained by EBUS-TBNA. Methods: Patients with proven NSCLC underwent EBUS-TBNA for the diagnosis of suspect lymph node metastasis, and cytological specimens were collected for epithelial cell culture and miRNA expression analysis. To validate the miRNA expression profile, we compared the results from EBUS-TBNA NSCLC specimens with those obtained from formalin-fixed paraffin-embedded (FFPE) mediastinoscopy specimens. Results: Analysis of the miRNA expression profiles of three independent EBUS-TBNA-derived primary cell lines allowed the screening of 377 different human miRNAs. One hundred and fifty miRNAs were detected in all cell lines. Analysis of the miRNA expression profile in mediastinoscopy specimens showed a strong similarity in the clusters analysed. Conclusions: The miRNA expression profile is feasible and reliable in EBUS-TBNA specimens. Validation of this protocol in fresh cytological specimens represents an effective and reproducible method to correlate translational and clinical research.
KW - Bronchoscopy
KW - Endobronchial ultrasound
KW - Non-small cell lung cancer (NSCLC)
KW - Translational research
UR - http://www.scopus.com/inward/record.url?scp=85041173436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85041173436&partnerID=8YFLogxK
U2 - 10.21037/jtd.2017.12.69
DO - 10.21037/jtd.2017.12.69
M3 - Article
AN - SCOPUS:85041173436
VL - 10
SP - 408
EP - 415
JO - Journal of Thoracic Disease
JF - Journal of Thoracic Disease
SN - 2072-1439
IS - 1
ER -