MicroRNA Expression Profiling in Psoriatic Arthritis

Andrea Pelosi, Claudio Lunardi, Piera Filomena Fiore, Elisa Tinazzi, Giuseppe Patuzzo, Giuseppe Argentino, Francesca Moretta, Antonio Puccetti, Marzia Dolcino

Research output: Contribution to journalArticle

Abstract

Background: Psoriatic arthritis (PsA) is an inflammatory arthritis, characterized by bone erosions and new bone formation. MicroRNAs (miRNAs) are key regulators of the immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in PsA is not fully elucidated. We aimed to identify miRNA expression signatures associated with PsA and to investigate their potential implication in the disease pathogenesis.

Methods: miRNA microarray was performed in blood cells of PsA patients and healthy controls. miRNA pathway analyses were performed and the global miRNA profiling was combined with transcriptome data in PsA. Deregulation of selected miRNAs was validated by real-time PCR.

Results: We identified specific miRNA signatures associated with PsA patients with active disease. These miRNAs target pathways relevant in PsA, such as TNF, MAPK, and WNT signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the PsA transcriptome. miR-126-3p was the most downregulated miRNA in active patients. Noteworthy, miR-126 overexpression induced a decreased expression of genes implicated in PsA.

Conclusions: This study sheds light on some epigenetic aspects of PsA identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in PsA.

Original languageEnglish
Pages (from-to)7305380
Number of pages15
JournalBioMed Research International
Volume2018
DOIs
Publication statusPublished - Apr 23 2018

Keywords

  • Adult
  • Arthritis, Psoriatic/blood
  • Biomarkers/blood
  • Female
  • Gene Expression Profiling
  • Humans
  • Jurkat Cells
  • Male
  • MicroRNAs/blood
  • Middle Aged
  • Signal Transduction/genetics
  • Transcriptome/genetics

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  • Cite this

    Pelosi, A., Lunardi, C., Fiore, P. F., Tinazzi, E., Patuzzo, G., Argentino, G., Moretta, F., Puccetti, A., & Dolcino, M. (2018). MicroRNA Expression Profiling in Psoriatic Arthritis. BioMed Research International, 2018, 7305380. https://doi.org/10.1155/2018/7305380