MicroRNA expression profiling in the prefrontal cortex: Putative mechanisms for the cognitive effects of adolescent high fat feeding

Marie A. Labouesse, Marcello Polesel, Elena Clementi, Flavia Müller, Enni Markkanen, Forouhar Mouttet, Annamaria Cattaneo, Juliet Richetto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The medial prefrontal cortex (mPFC), master regulator of higher-order cognitive functions, is the only brain region that matures until late adolescence. During this period, the mPFC is sensitive to stressful events or suboptimal nutrition. For instance, high-fat diet (HFD) feeding during adolescence markedly impairs prefrontal-dependent cognition. It also provokes multiple changes at the cellular and synaptic scales within the mPFC, suggesting that major transcriptional events are elicited by HFD during this maturational period. The nature of this transcriptional reprogramming remains unknown, but may include epigenetic processes, in particular microRNAs, known to directly regulate synaptic functions. We used high-throughput screening in the adolescent mouse mPFC and identified 38 microRNAs differentially regulated by HFD, in particular mir-30e-5p. We used a luciferase assay to confirm the functional effect of mir-30e-5p on a chosen target: Ephrin-A3. Using global pathway analyses of predicted microRNA targets, we identified biological pathways putatively affected by HFD. Axon guidance was the top-1 pathway, validated by identifying gene expression changes of axon guidance molecules following HFD. Our findings delineate major microRNA transcriptional reprogramming within the mPFC induced by adolescent HFD. These results will help understanding the contribution of microRNAs in the emergence of cognitive deficits following early-life environmental events.

Original languageEnglish
Article number8344
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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High Fat Diet
Prefrontal Cortex
MicroRNAs
Fats
Ephrin-A3
Cognition
Genetic Epigenesis
Luciferases
Gene Expression
Brain

ASJC Scopus subject areas

  • General

Cite this

Labouesse, M. A., Polesel, M., Clementi, E., Müller, F., Markkanen, E., Mouttet, F., ... Richetto, J. (2018). MicroRNA expression profiling in the prefrontal cortex: Putative mechanisms for the cognitive effects of adolescent high fat feeding. Scientific Reports, 8(1), [8344]. https://doi.org/10.1038/s41598-018-26631-x

MicroRNA expression profiling in the prefrontal cortex : Putative mechanisms for the cognitive effects of adolescent high fat feeding. / Labouesse, Marie A.; Polesel, Marcello; Clementi, Elena; Müller, Flavia; Markkanen, Enni; Mouttet, Forouhar; Cattaneo, Annamaria; Richetto, Juliet.

In: Scientific Reports, Vol. 8, No. 1, 8344, 01.12.2018.

Research output: Contribution to journalArticle

Labouesse, Marie A. ; Polesel, Marcello ; Clementi, Elena ; Müller, Flavia ; Markkanen, Enni ; Mouttet, Forouhar ; Cattaneo, Annamaria ; Richetto, Juliet. / MicroRNA expression profiling in the prefrontal cortex : Putative mechanisms for the cognitive effects of adolescent high fat feeding. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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