MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis

Catalin Vasilescu, Simona Rossi, Masayohi Shimizu, Stefan Tudor, Angelo Veronese, Manuela Ferracin, Milena S. Nicoloso, Elisa Barbarotto, Monica Popa, Oana Stanciulea, Michael H. Fernandez, Dan Tulbure, Carlos E. Bueso-Ramos, Massimo Negrini, George A. Calin

Research output: Contribution to journalArticle

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Abstract

Background: The physiopathology of sepsis continues to be poorly understood, and despite recent advances in its management, sepsis is still a life-threatening condition with a poor outcome. If new diagnostic markers related to sepsis pathogenesis will be identified, new specific therapies might be developed and mortality reduced. Small regulatory non-coding RNAs, microRNAs (miRNAs), were recently linked to various diseases; the aim of our prospective study was to identify miRNAs that can differentiate patients with early-stage sepsis from healthy controls and to determine if miRNA levels correlate with the severity assessed by the Sequential Organ Failure Assessment (SOFA) score. Methodology/Principal Findings: By using genome-wide miRNA profiling by microarray in peripheral blood leukocytes, we found that miR-150, miR-182, miR-342-5p, and miR-486 expression profiles differentiated sepsis patients from healthy controls. We also proved by quantitative reverse transcription-polymerase chain reaction that miR-150 levels were significantly reduced in plasma samples of sepsis patients and correlated with the level of disease severity measured by the SOFA score, but were independent of the white blood counts (WBC). We found that plasma levels of tumor necrosis factor alpha, interleukin-10, and interleukin-18, all genes with sequence complementarity to miR-150, were negatively correlated with the plasma levels of this miRNA. Furthermore, we identified that the plasma levels ratio for miR-150/interleukin-18 can be used for assessing the severity of the sepsis. Conclusions/Significance: We propose that miR-150 levels in both leukocytes and plasma correlate with the aggressiveness of sepsis and can be used as a marker of early sepsis. Furthermore, we envision miR-150 restoration as a future therapeutic option in sepsis patients.

Original languageEnglish
Article numbere7405
JournalPLoS One
Volume4
Issue number10
DOIs
Publication statusPublished - Oct 12 2009

Fingerprint

Dermatoglyphics
MicroRNAs
microRNA
Sepsis
Plasmas
interleukin-18
Interleukin-18
leukocytes
Blood
Genes
Organ Dysfunction Scores
therapeutics
Untranslated RNA
Polymerase chain reaction
blood
Transcription
Microarrays
interleukin-10
pathophysiology
prospective studies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Vasilescu, C., Rossi, S., Shimizu, M., Tudor, S., Veronese, A., Ferracin, M., ... Calin, G. A. (2009). MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis. PLoS One, 4(10), [e7405]. https://doi.org/10.1371/journal.pone.0007405

MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis. / Vasilescu, Catalin; Rossi, Simona; Shimizu, Masayohi; Tudor, Stefan; Veronese, Angelo; Ferracin, Manuela; Nicoloso, Milena S.; Barbarotto, Elisa; Popa, Monica; Stanciulea, Oana; Fernandez, Michael H.; Tulbure, Dan; Bueso-Ramos, Carlos E.; Negrini, Massimo; Calin, George A.

In: PLoS One, Vol. 4, No. 10, e7405, 12.10.2009.

Research output: Contribution to journalArticle

Vasilescu, C, Rossi, S, Shimizu, M, Tudor, S, Veronese, A, Ferracin, M, Nicoloso, MS, Barbarotto, E, Popa, M, Stanciulea, O, Fernandez, MH, Tulbure, D, Bueso-Ramos, CE, Negrini, M & Calin, GA 2009, 'MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis', PLoS One, vol. 4, no. 10, e7405. https://doi.org/10.1371/journal.pone.0007405
Vasilescu C, Rossi S, Shimizu M, Tudor S, Veronese A, Ferracin M et al. MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis. PLoS One. 2009 Oct 12;4(10). e7405. https://doi.org/10.1371/journal.pone.0007405
Vasilescu, Catalin ; Rossi, Simona ; Shimizu, Masayohi ; Tudor, Stefan ; Veronese, Angelo ; Ferracin, Manuela ; Nicoloso, Milena S. ; Barbarotto, Elisa ; Popa, Monica ; Stanciulea, Oana ; Fernandez, Michael H. ; Tulbure, Dan ; Bueso-Ramos, Carlos E. ; Negrini, Massimo ; Calin, George A. / MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis. In: PLoS One. 2009 ; Vol. 4, No. 10.
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AU - Veronese, Angelo

AU - Ferracin, Manuela

AU - Nicoloso, Milena S.

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AU - Popa, Monica

AU - Stanciulea, Oana

AU - Fernandez, Michael H.

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AU - Calin, George A.

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