microRNA levels in paraffin-embedded indolent B-cell non-Hodgkin lymphoma tissues from patients chronically infected with hepatitis B or C virus

Roberto Bruni, Cinzia Marcantonio, Alessandro Pulsoni, Paola Tataseo, Federico De Angelis, Enea Spada, Fabrizio Marcucci, Sara Panfilio, Paolo Bianco, Mara Riminucci, Umbertina Villano, Maria Elena Tosti, Anna Rita Ciccaglione, Alfonso Mele

Research output: Contribution to journalArticle

Abstract

Background: Epidemiological evidence links Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) to B-cell non-Hodgkin lymphoma (B-NHL). These B-NHLs, particularly those associated with HCV, may represent a distinct sub-group with peculiar molecular features, including peculiar expression of microRNAs (miRs). The aim of the present study was to search for miRs whose level in indolent B-NHL tissues could be associated with HBV or HCV infection. Methods: Fourteen formalin fixed paraffin embedded (FFPE) tissues from HBV+, HCV+ and HBV-/HCV- indolent B-NHL patients were analyzed for levels of 34 selected miRs by quantitative Real-Time PCR. Reactive lymph nodes (RLNs) from HBV-/HCV- patients were included as non-tumor control. Statistical analysis of output data included Pearson and Spearman correlation and Mann-Whitney test and were carried out by the STATA software. Results: MiR-92a was decreased exclusively in HBV-/HCV- B-NHLs, while miR-30b was increased in HBV+ and HCV+ samples, though only the HCV+ achieved full statistical significance. Analysis of a small subset of B-NHLs belonging to the same histological subtype (Nodal Marginal Zone Lymphoma) highlighted three miRs associated with HCV infection (miR-223, miR-29a and miR-29b) and confirmed decreased level of miR-92a in HBV-/HCV- samples also when considering this restricted B-NHL group. Conclusions: Although caution is needed due to the limited number of analyzed samples, overall the results suggest that differences at the miR expression level exist between indolent B-NHLs developed in patients with or without HBV or HCV infection. The identification of three further miRs associated with HCV by analyzing histologically homogeneous samples suggests that variations of miR levels possibly associated with HBV or HCV may be obscured by the tissue-specific variability of miR level associated with the different histological subtypes of B-NHL. Thus, the identification of further miRs will require, in addition to an increased sample size, the comparison of B-NHL tissues with the same histological classification.

Original languageEnglish
Article numberS6
JournalBMC Infectious Diseases
Volume14
Issue number5
DOIs
Publication statusPublished - Sep 5 2014

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B-Cell Lymphoma
MicroRNAs
Hepatitis B virus
Hepacivirus
Paraffin
Non-Hodgkin's Lymphoma
Virus Diseases
Statistical Data Interpretation
Sample Size
Formaldehyde
Real-Time Polymerase Chain Reaction
Lymphoma
Software
Lymph Nodes

Keywords

  • FFPE
  • Hepatitis B Virus
  • Hepatitis C Virus
  • Lymphoma
  • MicroRNA

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

microRNA levels in paraffin-embedded indolent B-cell non-Hodgkin lymphoma tissues from patients chronically infected with hepatitis B or C virus. / Bruni, Roberto; Marcantonio, Cinzia; Pulsoni, Alessandro; Tataseo, Paola; De Angelis, Federico; Spada, Enea; Marcucci, Fabrizio; Panfilio, Sara; Bianco, Paolo; Riminucci, Mara; Villano, Umbertina; Tosti, Maria Elena; Ciccaglione, Anna Rita; Mele, Alfonso.

In: BMC Infectious Diseases, Vol. 14, No. 5, S6, 05.09.2014.

Research output: Contribution to journalArticle

Bruni, R, Marcantonio, C, Pulsoni, A, Tataseo, P, De Angelis, F, Spada, E, Marcucci, F, Panfilio, S, Bianco, P, Riminucci, M, Villano, U, Tosti, ME, Ciccaglione, AR & Mele, A 2014, 'microRNA levels in paraffin-embedded indolent B-cell non-Hodgkin lymphoma tissues from patients chronically infected with hepatitis B or C virus', BMC Infectious Diseases, vol. 14, no. 5, S6. https://doi.org/10.1186/1471-2334-14-S5-S6
Bruni, Roberto ; Marcantonio, Cinzia ; Pulsoni, Alessandro ; Tataseo, Paola ; De Angelis, Federico ; Spada, Enea ; Marcucci, Fabrizio ; Panfilio, Sara ; Bianco, Paolo ; Riminucci, Mara ; Villano, Umbertina ; Tosti, Maria Elena ; Ciccaglione, Anna Rita ; Mele, Alfonso. / microRNA levels in paraffin-embedded indolent B-cell non-Hodgkin lymphoma tissues from patients chronically infected with hepatitis B or C virus. In: BMC Infectious Diseases. 2014 ; Vol. 14, No. 5.
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T1 - microRNA levels in paraffin-embedded indolent B-cell non-Hodgkin lymphoma tissues from patients chronically infected with hepatitis B or C virus

AU - Bruni, Roberto

AU - Marcantonio, Cinzia

AU - Pulsoni, Alessandro

AU - Tataseo, Paola

AU - De Angelis, Federico

AU - Spada, Enea

AU - Marcucci, Fabrizio

AU - Panfilio, Sara

AU - Bianco, Paolo

AU - Riminucci, Mara

AU - Villano, Umbertina

AU - Tosti, Maria Elena

AU - Ciccaglione, Anna Rita

AU - Mele, Alfonso

PY - 2014/9/5

Y1 - 2014/9/5

N2 - Background: Epidemiological evidence links Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) to B-cell non-Hodgkin lymphoma (B-NHL). These B-NHLs, particularly those associated with HCV, may represent a distinct sub-group with peculiar molecular features, including peculiar expression of microRNAs (miRs). The aim of the present study was to search for miRs whose level in indolent B-NHL tissues could be associated with HBV or HCV infection. Methods: Fourteen formalin fixed paraffin embedded (FFPE) tissues from HBV+, HCV+ and HBV-/HCV- indolent B-NHL patients were analyzed for levels of 34 selected miRs by quantitative Real-Time PCR. Reactive lymph nodes (RLNs) from HBV-/HCV- patients were included as non-tumor control. Statistical analysis of output data included Pearson and Spearman correlation and Mann-Whitney test and were carried out by the STATA software. Results: MiR-92a was decreased exclusively in HBV-/HCV- B-NHLs, while miR-30b was increased in HBV+ and HCV+ samples, though only the HCV+ achieved full statistical significance. Analysis of a small subset of B-NHLs belonging to the same histological subtype (Nodal Marginal Zone Lymphoma) highlighted three miRs associated with HCV infection (miR-223, miR-29a and miR-29b) and confirmed decreased level of miR-92a in HBV-/HCV- samples also when considering this restricted B-NHL group. Conclusions: Although caution is needed due to the limited number of analyzed samples, overall the results suggest that differences at the miR expression level exist between indolent B-NHLs developed in patients with or without HBV or HCV infection. The identification of three further miRs associated with HCV by analyzing histologically homogeneous samples suggests that variations of miR levels possibly associated with HBV or HCV may be obscured by the tissue-specific variability of miR level associated with the different histological subtypes of B-NHL. Thus, the identification of further miRs will require, in addition to an increased sample size, the comparison of B-NHL tissues with the same histological classification.

AB - Background: Epidemiological evidence links Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) to B-cell non-Hodgkin lymphoma (B-NHL). These B-NHLs, particularly those associated with HCV, may represent a distinct sub-group with peculiar molecular features, including peculiar expression of microRNAs (miRs). The aim of the present study was to search for miRs whose level in indolent B-NHL tissues could be associated with HBV or HCV infection. Methods: Fourteen formalin fixed paraffin embedded (FFPE) tissues from HBV+, HCV+ and HBV-/HCV- indolent B-NHL patients were analyzed for levels of 34 selected miRs by quantitative Real-Time PCR. Reactive lymph nodes (RLNs) from HBV-/HCV- patients were included as non-tumor control. Statistical analysis of output data included Pearson and Spearman correlation and Mann-Whitney test and were carried out by the STATA software. Results: MiR-92a was decreased exclusively in HBV-/HCV- B-NHLs, while miR-30b was increased in HBV+ and HCV+ samples, though only the HCV+ achieved full statistical significance. Analysis of a small subset of B-NHLs belonging to the same histological subtype (Nodal Marginal Zone Lymphoma) highlighted three miRs associated with HCV infection (miR-223, miR-29a and miR-29b) and confirmed decreased level of miR-92a in HBV-/HCV- samples also when considering this restricted B-NHL group. Conclusions: Although caution is needed due to the limited number of analyzed samples, overall the results suggest that differences at the miR expression level exist between indolent B-NHLs developed in patients with or without HBV or HCV infection. The identification of three further miRs associated with HCV by analyzing histologically homogeneous samples suggests that variations of miR levels possibly associated with HBV or HCV may be obscured by the tissue-specific variability of miR level associated with the different histological subtypes of B-NHL. Thus, the identification of further miRs will require, in addition to an increased sample size, the comparison of B-NHL tissues with the same histological classification.

KW - FFPE

KW - Hepatitis B Virus

KW - Hepatitis C Virus

KW - Lymphoma

KW - MicroRNA

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