microRNA-mRNA network model in patients with achalasia

Orazio Palmieri, Tommaso Mazza, Gabrio Bassotti, Antonio Merla, Salvatore Tolone, Tommaso Biagini, Antonello Cuttitta, Fabrizio Bossa, Giuseppina Martino, Tiziana Latiano, Giuseppe Corritore, Domenica Gioffreda, Orazio Palumbo, Massimo Carella, Anna Panza, Angelo Andriulli, Anna Latiano

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Achalasia is a rare idiopathic disease with a complex etio-pathogenesis still unknown. This study aimed to identify microRNA (miRNA)-mRNA regulatory networks underlying achalasia. Methods: The investigation was performed in tissue specimens from 11 patients and five controls using the microarray technology followed by an integrated bioinformatics analysis. Key Results: One hundred and six miRNAs were significantly up-regulated and 64 were down-regulated in achalasia patients. The expression of the most 10 differential expressed miRNAs (miR-122-5p, miR-133a-3p, miR-504-5p, miR-187-3p, miR-133b, miR-200c-3p, miR-375, miR-200b-5p, miR-200b-3p, and miR203a) was confirmed by droplet digital PCR in an independent cohort. The interactions between the significant miRNAs and their targets uncovered 14 miRNA-mRNA interacting pairs with experimentally predicted genes (ie, FN1, ROCK2, DPYSL2), and 35 pairs with not experimentally target genes (ie, SULF1, MRVI1, PRKG1); all genes were involved in immune cell trafficking, skeletal and muscular system development, nervous system development macro-processes. Conclusion & Inferences: The mRNA–miRNA regulatory networks described in this study provide new insights in the genetic background of the disease, suggesting further investigations in novel pathogenic mechanisms.

Original languageEnglish
Article numbere13764
JournalNeurogastroenterology and Motility
DOIs
Publication statusAccepted/In press - Jan 1 2019

Keywords

  • achalasia
  • expression profile
  • microRNA
  • mRNA

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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