MicroRNA networks regulated by all-trans retinoic acid and Lapatinib control the growth, survival and motility of breast cancer cells

James Neil Fisher, Mineko Terao, Maddalena Fratelli, Mami Kurosaki, Gabriela Paroni, Adriana Zanetti, Maurizio Gianni, Marco Bolis, Monica Lupi, Anna Tsykin, Gregory J. Goodall, Enrico Garattini

Research output: Contribution to journalArticle

Abstract

SKBR3-cells, characterized by ERBB2/RARA co-amplification, represent a subgroup of HER2+ breast-cancers sensitive to all-trans retinoic acid (ATRA) and Lapatinib. In this model, the two agents alone or in combination modulate the expression of 174 microRNAs (miRs). These miRs and predicted target-transcripts are organized in four interconnected modules (Module-1 to-4). Module-1 and Module-3 consist of ATRA/Lapatinib up-regulated and potentially anti-oncogenic miRs, while Module-2 contains ATRA/Lapatinib down-regulated and potentially pro-oncogenic miRs. Consistent with this, the expression levels of Module-1/-3 and Module-2 miRs are higher and lower, respectively, in normal mammary tissues relative to ductal-carcinoma-in-situ, invasive-ductal-carcinoma and metastases. This indicates associations between tumor-progression and the expression profiles of Module-1 to-3 miRs. Similar associations are observed with tumor proliferation-scores, staging, size and overall-survival using TCGA (The Cancer Genome Atlas) data. Forced expression of Module-1 miRs, (miR-29a-3p; miR-874-3p) inhibit SKBR3-cell growth and Module-3 miRs (miR-575; miR-1225-5p) reduce growth and motility. Module-2 miRs (miR-125a; miR-193; miR-210) increase SKBR3 cell growth, survival and motility. Some of these effects are of general significance, being replicated in other breast cancer cell lines representing the heterogeneity of this disease. Finally, our study demonstrates that HIPK2-kinase and the PLCXD1-phospholipase-C are novel targets of miR-193a-5p/miR-210-3p and miR-575/miR-1225-5p, respectively.

Original languageEnglish
Pages (from-to)13176-13200
Number of pages25
JournalOncotarget
Volume6
Issue number15
Publication statusPublished - 2015

Fingerprint

Tretinoin
MicroRNAs
Breast Neoplasms
Growth
lapatinib
Neoplasms
Ductal Carcinoma
Carcinoma, Intraductal, Noninfiltrating
Atlases
Type C Phospholipases
Cell Movement
Cell Survival
Breast
Phosphotransferases
Genome
Neoplasm Metastasis
Cell Line

Keywords

  • Breast cancer
  • Lapatinib
  • MicroRNA
  • Network analysis
  • Retinoic acid

ASJC Scopus subject areas

  • Oncology

Cite this

MicroRNA networks regulated by all-trans retinoic acid and Lapatinib control the growth, survival and motility of breast cancer cells. / Fisher, James Neil; Terao, Mineko; Fratelli, Maddalena; Kurosaki, Mami; Paroni, Gabriela; Zanetti, Adriana; Gianni, Maurizio; Bolis, Marco; Lupi, Monica; Tsykin, Anna; Goodall, Gregory J.; Garattini, Enrico.

In: Oncotarget, Vol. 6, No. 15, 2015, p. 13176-13200.

Research output: Contribution to journalArticle

Fisher, James Neil ; Terao, Mineko ; Fratelli, Maddalena ; Kurosaki, Mami ; Paroni, Gabriela ; Zanetti, Adriana ; Gianni, Maurizio ; Bolis, Marco ; Lupi, Monica ; Tsykin, Anna ; Goodall, Gregory J. ; Garattini, Enrico. / MicroRNA networks regulated by all-trans retinoic acid and Lapatinib control the growth, survival and motility of breast cancer cells. In: Oncotarget. 2015 ; Vol. 6, No. 15. pp. 13176-13200.
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AU - Kurosaki, Mami

AU - Paroni, Gabriela

AU - Zanetti, Adriana

AU - Gianni, Maurizio

AU - Bolis, Marco

AU - Lupi, Monica

AU - Tsykin, Anna

AU - Goodall, Gregory J.

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