MicroRNA profiles in familial and sporadic medullary thyroid carcinoma: Preliminary relationships with RET status and outcome

Caterina Mian, Gianmaria Pennelli, Matteo Fassan, Mariangela Balistreri, Susi Barollo, Elisabetta Cavedon, Francesca Galuppini, Marco Pizzi, Federica Vianello, Maria Rosa Pelizzo, Maria Elisa Girelli, Massimo Rugge, Giuseppe Opocher

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Abstract

Background: MicroRNAs (miRNAs) are involved in the pathogenesis of human cancers, including medullary thyroid carcinoma (MTC). The aim of this study was to test the hypothesis that different miRNA profiles are related to RET status and prognosis in patients with hereditary MTC (hMTC) and sporadic MTC (sMTC). Methods: We analyzed the expression of nine miRNAs (miR-21, miR-127, miR-154, miR-224, miR-323, miR-370, miR-9*, miR-183, and miR-375) by quantitative real-time-polymerase chain reaction in 34 cases of sMTC, 6 cases of hMTC, and 2 cases of C-cell hyperplasia (CCH). We also analyzed the immunohistochemical expression of PDCD4, an miR-21 gene target. sMTC (n=34) was genotyped for somatic RET and RAS mutations. Disease status was defined on the basis of the concentration of serum calcitonin at the latest follow-up and other parameters as indicated in the results. Results: MTC and CCH were both characterized by a significant overexpression of the whole set of miRNAs (the increase being 4.2-fold for miR-21, 6.7-fold for miR-127, 8.8-fold for miR-154, 6.6-fold for miR-224, 5.8-fold for miR-323, 6.1-fold for miR-370, 13-fold for miR-9*, 6.7-fold for miR-183, and 10.1 for miR-375, p

Original languageEnglish
Pages (from-to)890-896
Number of pages7
JournalThyroid
Volume22
Issue number9
DOIs
Publication statusPublished - Sep 1 2012

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ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Mian, C., Pennelli, G., Fassan, M., Balistreri, M., Barollo, S., Cavedon, E., Galuppini, F., Pizzi, M., Vianello, F., Pelizzo, M. R., Girelli, M. E., Rugge, M., & Opocher, G. (2012). MicroRNA profiles in familial and sporadic medullary thyroid carcinoma: Preliminary relationships with RET status and outcome. Thyroid, 22(9), 890-896. https://doi.org/10.1089/thy.2012.0045