MicroRNA profiling in human medulloblastoma

Elisabetta Ferretti, Enrico De Smaele, Agnese Po, Lucia Di Marcotullio, Emanuele Tosi, Maria Salome B Espinola, Concezio Di Rocco, Riccardo Riccardi, Felice Giangaspero, Alessio Farcomeni, Italo Nofroni, Pietro Laneve, Ubaldo Gioia, Elisa Caffarelli, Irene Bozzoni, Isabella Screpanti, Alberto Gulino

Research output: Contribution to journalArticle

Abstract

Medulloblastoma is an aggressive brain malignancy with high incidence in childhood. Current treatment approaches have limited efficacy and severe side effects. Therefore, new risk-adapted therapeutic strategies based on molecular classification are required. MicroRNA expression analysis has emerged as a powerful tool to identify candidate molecules playing an important role in a large number of malignancies. However, no data are yet available on human primary medulloblastomas. A high throughput microRNA expression profiles was performed in human primary medulloblastoma specimens to investigate microRNA involvement in medulloblastoma carcinogenesis. We identified specific microRNA expression patterns which distinguish medulloblastoma differing in histotypes (anaplastic, classic and desmoplastic), in molecular features (ErbB2 or c-Myc overexpressing tumors) and in disease-risk stratification. MicroRNAs expression profile clearly differentiates medulloblastoma from either adult or fetal normal cerebellar tissues. Only a few microRNAs displayed upregulated expression, while most of them were downregulated in tumor samples, suggesting a tumor growth-inhibitory function. This property has been addressed for miR-9 and miR-125a, whose rescued expression promoted medulloblastoma cell growth arrest and apoptosis while targeting the proproliferative truncated TrkC iso-form. In conclusion, misregulated microRNA expression profiles characterize human medulloblastomas, and may provide potential targets for novel therapeutic strategies.

Original languageEnglish
Pages (from-to)568-577
Number of pages10
JournalInternational Journal of Cancer
Volume124
Issue number3
DOIs
Publication statusPublished - Feb 1 2009

Keywords

  • Medulloblastoma
  • MicroRNA
  • MiRNA-125a
  • MiRNA-9

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

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  • Cite this

    Ferretti, E., De Smaele, E., Po, A., Marcotullio, L. D., Tosi, E., Espinola, M. S. B., Rocco, C. D., Riccardi, R., Giangaspero, F., Farcomeni, A., Nofroni, I., Laneve, P., Gioia, U., Caffarelli, E., Bozzoni, I., Screpanti, I., & Gulino, A. (2009). MicroRNA profiling in human medulloblastoma. International Journal of Cancer, 124(3), 568-577. https://doi.org/10.1002/ijc.23948