MicroRNA profiling in ovarian cancer

Marilena V. Iorio, Carlo M. Croce

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The evaluation of microRNA profiles has represented one of the first approaches to investigate the aberrant microRNA expression in different human cancers, providing the first experimental evidence of the involvement of these small noncoding RNAs in the tumorigenic process. Currently, these and other methods have been applied to the search of new molecular biomarkers of diagnosis, prognosis, and response to therapy prediction. Here, we described the approach we used for the determination of a miRNA signature unique for human ovarian cancer, first performing a large-scale screening using a custom-made microarray platform, and then validating the obtained data by Northern blot or real-time PCR.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages187-197
Number of pages11
Volume1049
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1049
ISSN (Print)10643745

Keywords

  • Biomarker
  • Diagnosis
  • Expression profiling
  • MicroRNAs
  • Ovarian cancer
  • Signature

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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