MicroRNA profiling reveals that miR-21, miR486 and miR-214 are upregulated and involved in cell survival in Sézary syndrome

M. G. Narducci, D. Arcelli, M. C. Picchio, C. Lazzeri, E. Pagani, F. Sampogna, E. Scala, P. Fadda, C. Cristofoletti, A. Facchiano, M. Frontani, A. Monopoli, M. Ferracin, M. Negrini, G. A. Lombardo, E. Caprini, G. Russo

Research output: Contribution to journalArticle

Abstract

Sezary syndrome (SS) is an incurable leukemic variant of cutaneous T-cell lymphoma and its pathogenesis is still unknown. Diagnosis/prognosis may strongly ameliorate the management of SS individuals. Here, we profiled the expression of 470 microRNAs (miRNAs) in a cohort of 22 SS patients, and we identified 45 miRNAs differentially expressed between SS and controls. Using predictive analysis, a list of 19 miRNAs, including miR-21, miR-214, miR-486, miR-18a, miR-342, miR-31 and let-7 members were also found. Moreover, we defined a signature of 14 miRNAs including again miR-21, potentially able to discriminate patients with unfavorable and favorable outcome. We validated our data for miR-21, miR-214 and miR-486 by qRTPCR, including an additional set of array-independent SS cases. In addition, we also provide an in vitro evidence for a contribution of miR-214, miR-486 and miR-21 to apoptotic resistance of CTCL cell line.

Original languageEnglish
Article numbere151
JournalCell Death and Disease
Volume2
Issue number4
DOIs
Publication statusPublished - Apr 2011

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Keywords

  • Diagnostic and prognostic markers
  • MicroRNAs
  • Sezary syndrome

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Cancer Research
  • Cellular and Molecular Neuroscience

Cite this

Narducci, M. G., Arcelli, D., Picchio, M. C., Lazzeri, C., Pagani, E., Sampogna, F., Scala, E., Fadda, P., Cristofoletti, C., Facchiano, A., Frontani, M., Monopoli, A., Ferracin, M., Negrini, M., Lombardo, G. A., Caprini, E., & Russo, G. (2011). MicroRNA profiling reveals that miR-21, miR486 and miR-214 are upregulated and involved in cell survival in Sézary syndrome. Cell Death and Disease, 2(4), [e151]. https://doi.org/10.1038/cddis.2011.32