MicroRNA signatures in human ovarian cancer

Marilena V. Iorio, Rosa Visone, Gianpiero Di Leva, Valentina Donati, Fabio Petrocca, Patrizia Casalini, Cristian Taccioli, Stefano Volinia, Chang Gong Liu, Hansjuerg Alder, George A. Calin, Sylvie Ménard, Carlo M. Croce

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Abstract

Epithelial ovarian cancer (EOC) is the sixth most common cancer in women worldwide and, despite advances in detection and therapies, it still represents the most lethal gynecologic malignancy in the industrialized countries. Unfortunately, still relatively little is known about the molecular events that lead to the development of this highly aggressive disease. The relatively recent discovery of micro-RNAs (miRNA), a class of small noncoding RNAs targeting multiple mRNAs and triggering translation repression and/or RNA degradation, has revealed the existence of a new level of gene expression regulation. Multiple studies involving various types of human cancers proved that miRNAs have a causal role in tumorigenesis. Here we show that, in comparison to normal ovary, miRNAs are aberrantly expressed in human ovarian cancer. The overall miRNA expression could clearly separate normal versus cancer tissues. The most significantly overexpressed miRNAs were miR-200a, miR-141, miR-200c, and miR-200b, whereas miR-199a, miR-140, miR-145, and miR-125b1 were among the most down-modulated miRNAs. We could also identify miRNAs whose expression was correlated with specific ovarian cancer biopathologic features, such as histotype, lymphovascular and organ invasion, and involvement of ovarian surface. Moreover, the levels of miR-21, miR-203, and miR-205, up-modulated in ovarian carcinomas compared with normal tissues, were significantly increased after 5-aza-2′-deoxycytidine demethylating treatment of OVCAR3 cells, suggesting that the DNA hypomethylation could be the mechanism responsible for their overexpression. Our results indicate that miRNAs might play a role in the pathogenesis of human EOC and identify altered miRNA gene methylation as a possible epigenetic mechanism involved in their aberrant expression.

Original languageEnglish
Pages (from-to)8699-8707
Number of pages9
JournalCancer Research
Volume67
Issue number18
DOIs
Publication statusPublished - Sep 15 2007

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MicroRNAs
Ovarian Neoplasms
decitabine
Neoplasms
Small Untranslated RNA
RNA Stability
Gene Expression Regulation
Protein Biosynthesis
Developed Countries
Epigenomics
Methylation
Ovary
Carcinogenesis
Carcinoma
DNA
Therapeutics
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Iorio, M. V., Visone, R., Di Leva, G., Donati, V., Petrocca, F., Casalini, P., ... Croce, C. M. (2007). MicroRNA signatures in human ovarian cancer. Cancer Research, 67(18), 8699-8707. https://doi.org/10.1158/0008-5472.CAN-07-1936

MicroRNA signatures in human ovarian cancer. / Iorio, Marilena V.; Visone, Rosa; Di Leva, Gianpiero; Donati, Valentina; Petrocca, Fabio; Casalini, Patrizia; Taccioli, Cristian; Volinia, Stefano; Liu, Chang Gong; Alder, Hansjuerg; Calin, George A.; Ménard, Sylvie; Croce, Carlo M.

In: Cancer Research, Vol. 67, No. 18, 15.09.2007, p. 8699-8707.

Research output: Contribution to journalArticle

Iorio, MV, Visone, R, Di Leva, G, Donati, V, Petrocca, F, Casalini, P, Taccioli, C, Volinia, S, Liu, CG, Alder, H, Calin, GA, Ménard, S & Croce, CM 2007, 'MicroRNA signatures in human ovarian cancer', Cancer Research, vol. 67, no. 18, pp. 8699-8707. https://doi.org/10.1158/0008-5472.CAN-07-1936
Iorio MV, Visone R, Di Leva G, Donati V, Petrocca F, Casalini P et al. MicroRNA signatures in human ovarian cancer. Cancer Research. 2007 Sep 15;67(18):8699-8707. https://doi.org/10.1158/0008-5472.CAN-07-1936
Iorio, Marilena V. ; Visone, Rosa ; Di Leva, Gianpiero ; Donati, Valentina ; Petrocca, Fabio ; Casalini, Patrizia ; Taccioli, Cristian ; Volinia, Stefano ; Liu, Chang Gong ; Alder, Hansjuerg ; Calin, George A. ; Ménard, Sylvie ; Croce, Carlo M. / MicroRNA signatures in human ovarian cancer. In: Cancer Research. 2007 ; Vol. 67, No. 18. pp. 8699-8707.
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