MicroRNAs in cancer chemoprevention: Method to isolate them from fresh tissues

Federica Ganci, Giovanni Blandino

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

microRNAs are 22-nucleotide-long double-strand small RNAs, able to modulate gene expression at posttranscriptional level, degrading mRNA and/or impairing translation. They have been shown to regulate mRNA and protein abundance and to participate in many regulatory circuits controlling developmental timing, cell proliferation and differentiation, apoptosis and stress response. Notably, microRNA activity has been correlated to the pathogenesis of cancer; they are aberrantly expressed in solid and hematological tumors, suggesting that they could function as oncogenes or tumor suppressors. The emerging role of miRNAs in the carcinogenesis and tumor progression has provided opportunities for their clinical application in the capacity of cancer detection, diagnosis, and prognosis prediction. Here, we describe the experimen­tal protocol used to isolate microRNAs from human tissues coming from head and neck, mesothelioma, and thymoma tumors in order to perform microarray and RT-qPCR experiments.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages21-29
Number of pages9
Volume1379
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1379
ISSN (Print)10643745

Keywords

  • Fresh tissues
  • HNSCC
  • Mesothelioma
  • MicroRNA
  • RNA extraction
  • Thymoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Ganci, F., & Blandino, G. (2016). MicroRNAs in cancer chemoprevention: Method to isolate them from fresh tissues. In Methods in Molecular Biology (Vol. 1379, pp. 21-29). (Methods in Molecular Biology; Vol. 1379). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3191-0_3