Microscopic in vivo description of cellular architecture of dermoscopic pigment network in nevi and melanomas

Giovanni Pellacani, Anna Maria Cesinaro, Caterina Longo, Costantino Grana, Stefania Seidenari

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Objective: To characterize the microscopic aspects of the dermoscopic pigment network in vivo, by means of confocal scanning laser microscopy. Design: Confocal imaging was performed on melanocytic lesions characterized by pigment network at dermoscopy. Some confocal architectural and cytologic features, as observed at the dermoepidermal junction, were morphologically described and quantified by means of a dedicated program. Setting: University medical department. Study Population: We studied confocal images of 15 melanomas, 15 dermoscopic atypical nevi, and 15 common nevi. Main Outcome Measures: Features referring to aspect, size, regularity, homogeneity, and infiltration of dermal papillae and to cellular size, regularity, and atypia were described by 2 observers on confocal images. Mean dermal papillary diameter, mean cell area, and shape irregularity were quantified by drawing papillae and cell contours on confocal images and measured with the use of a computer program. Results: Pigment network in melanomas consisted of large basal cells that circumscribed small to medium-sized dermal papillae with marked cellular atypia, sometimes infiltrating dermal papillae. On the other hand, common acquired nevi were characterized by lack of atypical cells and edged dermal papillae. Atypical nevi presented intermediate characteristics between clearly benign and malignant lesions. Conclusion: Cellular atypia was the most sensitive feature for melanoma diagnosis, whereas the presence of nucleated cells infiltrating dermal papillae was the most specific one.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalArchives of Dermatology
Volume141
Issue number2
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Nevi and Melanomas
Nevus
Skin
Melanoma
Dermoscopy
Cell Shape
Confocal Microscopy
Software
Outcome Assessment (Health Care)
Population

ASJC Scopus subject areas

  • Dermatology

Cite this

Microscopic in vivo description of cellular architecture of dermoscopic pigment network in nevi and melanomas. / Pellacani, Giovanni; Cesinaro, Anna Maria; Longo, Caterina; Grana, Costantino; Seidenari, Stefania.

In: Archives of Dermatology, Vol. 141, No. 2, 02.2005, p. 147-154.

Research output: Contribution to journalArticle

Pellacani, Giovanni ; Cesinaro, Anna Maria ; Longo, Caterina ; Grana, Costantino ; Seidenari, Stefania. / Microscopic in vivo description of cellular architecture of dermoscopic pigment network in nevi and melanomas. In: Archives of Dermatology. 2005 ; Vol. 141, No. 2. pp. 147-154.
@article{33d58639833247739571aa1df95e6f7f,
title = "Microscopic in vivo description of cellular architecture of dermoscopic pigment network in nevi and melanomas",
abstract = "Objective: To characterize the microscopic aspects of the dermoscopic pigment network in vivo, by means of confocal scanning laser microscopy. Design: Confocal imaging was performed on melanocytic lesions characterized by pigment network at dermoscopy. Some confocal architectural and cytologic features, as observed at the dermoepidermal junction, were morphologically described and quantified by means of a dedicated program. Setting: University medical department. Study Population: We studied confocal images of 15 melanomas, 15 dermoscopic atypical nevi, and 15 common nevi. Main Outcome Measures: Features referring to aspect, size, regularity, homogeneity, and infiltration of dermal papillae and to cellular size, regularity, and atypia were described by 2 observers on confocal images. Mean dermal papillary diameter, mean cell area, and shape irregularity were quantified by drawing papillae and cell contours on confocal images and measured with the use of a computer program. Results: Pigment network in melanomas consisted of large basal cells that circumscribed small to medium-sized dermal papillae with marked cellular atypia, sometimes infiltrating dermal papillae. On the other hand, common acquired nevi were characterized by lack of atypical cells and edged dermal papillae. Atypical nevi presented intermediate characteristics between clearly benign and malignant lesions. Conclusion: Cellular atypia was the most sensitive feature for melanoma diagnosis, whereas the presence of nucleated cells infiltrating dermal papillae was the most specific one.",
author = "Giovanni Pellacani and Cesinaro, {Anna Maria} and Caterina Longo and Costantino Grana and Stefania Seidenari",
year = "2005",
month = "2",
doi = "10.1001/archderm.141.2.147",
language = "English",
volume = "141",
pages = "147--154",
journal = "Archives of Dermatology",
issn = "0003-987X",
publisher = "American Medical Association",
number = "2",

}

TY - JOUR

T1 - Microscopic in vivo description of cellular architecture of dermoscopic pigment network in nevi and melanomas

AU - Pellacani, Giovanni

AU - Cesinaro, Anna Maria

AU - Longo, Caterina

AU - Grana, Costantino

AU - Seidenari, Stefania

PY - 2005/2

Y1 - 2005/2

N2 - Objective: To characterize the microscopic aspects of the dermoscopic pigment network in vivo, by means of confocal scanning laser microscopy. Design: Confocal imaging was performed on melanocytic lesions characterized by pigment network at dermoscopy. Some confocal architectural and cytologic features, as observed at the dermoepidermal junction, were morphologically described and quantified by means of a dedicated program. Setting: University medical department. Study Population: We studied confocal images of 15 melanomas, 15 dermoscopic atypical nevi, and 15 common nevi. Main Outcome Measures: Features referring to aspect, size, regularity, homogeneity, and infiltration of dermal papillae and to cellular size, regularity, and atypia were described by 2 observers on confocal images. Mean dermal papillary diameter, mean cell area, and shape irregularity were quantified by drawing papillae and cell contours on confocal images and measured with the use of a computer program. Results: Pigment network in melanomas consisted of large basal cells that circumscribed small to medium-sized dermal papillae with marked cellular atypia, sometimes infiltrating dermal papillae. On the other hand, common acquired nevi were characterized by lack of atypical cells and edged dermal papillae. Atypical nevi presented intermediate characteristics between clearly benign and malignant lesions. Conclusion: Cellular atypia was the most sensitive feature for melanoma diagnosis, whereas the presence of nucleated cells infiltrating dermal papillae was the most specific one.

AB - Objective: To characterize the microscopic aspects of the dermoscopic pigment network in vivo, by means of confocal scanning laser microscopy. Design: Confocal imaging was performed on melanocytic lesions characterized by pigment network at dermoscopy. Some confocal architectural and cytologic features, as observed at the dermoepidermal junction, were morphologically described and quantified by means of a dedicated program. Setting: University medical department. Study Population: We studied confocal images of 15 melanomas, 15 dermoscopic atypical nevi, and 15 common nevi. Main Outcome Measures: Features referring to aspect, size, regularity, homogeneity, and infiltration of dermal papillae and to cellular size, regularity, and atypia were described by 2 observers on confocal images. Mean dermal papillary diameter, mean cell area, and shape irregularity were quantified by drawing papillae and cell contours on confocal images and measured with the use of a computer program. Results: Pigment network in melanomas consisted of large basal cells that circumscribed small to medium-sized dermal papillae with marked cellular atypia, sometimes infiltrating dermal papillae. On the other hand, common acquired nevi were characterized by lack of atypical cells and edged dermal papillae. Atypical nevi presented intermediate characteristics between clearly benign and malignant lesions. Conclusion: Cellular atypia was the most sensitive feature for melanoma diagnosis, whereas the presence of nucleated cells infiltrating dermal papillae was the most specific one.

UR - http://www.scopus.com/inward/record.url?scp=13444249535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13444249535&partnerID=8YFLogxK

U2 - 10.1001/archderm.141.2.147

DO - 10.1001/archderm.141.2.147

M3 - Article

VL - 141

SP - 147

EP - 154

JO - Archives of Dermatology

JF - Archives of Dermatology

SN - 0003-987X

IS - 2

ER -