Microsomal enzyme activity in perfused rat liver

I. Bartošek, A. Guaitani, S. Garattini, I. Simonazzi

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The activities of microsomal enzymes were observed during perfusion of livers isolated from both normal and phenobarbital treated rats. The hydroxylation of aniline and the O-demethylation of p-nitroanisol did not decrease substantially (by 10 per cent only) in the 9000 g fraction prepared from livers perfused for 4 hr. N-demethylation of aminopyrine was reduced by 60 per cent in the same experimental conditions. A similar decrease of N-demethylation was also observed when livers were isolated from animals treated with phenobarbital. The content of cytochrome P-450 in the microsomes was stable during a 4-hr perfusion of isolated liver. The decrease of N-demethylation of aminopyrine could be partly restored by the simultaneous infusion of nicotinic acid, which is a precursor of NAD and NADP biosynthesis.

Original languageEnglish
Pages (from-to)599-605
Number of pages7
JournalBiochemical Pharmacology
Issue number3
Publication statusPublished - Feb 1 1974

ASJC Scopus subject areas

  • Pharmacology


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