Abstract
Arrhythmogenesis during heart failure is a major clinical problem. Regional electrical gradients produce arrhythmias, and cellular ionic transmembrane gradients are its originators. We investigated whether the nanoscale mechanosensitive properties of cardiomyocytes from failing hearts have a bearing upon the initiation of abnormal electrical activity. Hydrojets through a nanopipette indent specific locations on the sarcolemma and initiate intracellular calcium release in both healthy and heart failure cardiomyocytes, as well as in human failing cardiomyocytes. In healthy cells, calcium is locally confined, whereas in failing cardiomyocytes, calcium propagates. Heart failure progressively stiffens the membrane and displaces sub-sarcolemmal mitochondria. Colchicine in healthy cells mimics the failing condition by stiffening the cells, disrupting microtubules, shifting mitochondria, and causing calcium release. Uncoupling the mitochondrial proton gradient abolished calcium initiation in both failing and colchicine-treated cells. We propose the disruption of microtubule-dependent mitochondrial mechanosensor microdomains as a mechanism for abnormal calcium release in failing heart. Miragoli et al. show that failing heart cells have altered sensitivity to nanomechanical stimuli mediated by changes in the alignment of microtubules. The microtubule network disorganization leads to displacement of mitochondria and alterations in calcium release.
| Original language | English |
|---|---|
| Pages (from-to) | 140-151 |
| Number of pages | 12 |
| Journal | Cell Reports |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 5 2016 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Microtubule-Dependent Mitochondria Alignment Regulates Calcium Release in Response to Nanomechanical Stimulus in Heart Myocytes. / Miragoli, Michele; Sanchez-Alonso, Jose L.; Bhargava, Anamika; Wright, Peter T.; Sikkel, Markus; Schobesberger, Sophie; Diakonov, Ivan; Novak, Pavel; Castaldi, Alessandra; Cattaneo, Paola; Lyon, Alexander R.; Lab, Max J.; Gorelik, Julia.
In: Cell Reports, Vol. 14, No. 1, 05.01.2016, p. 140-151.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microtubule-Dependent Mitochondria Alignment Regulates Calcium Release in Response to Nanomechanical Stimulus in Heart Myocytes
AU - Miragoli, Michele
AU - Sanchez-Alonso, Jose L.
AU - Bhargava, Anamika
AU - Wright, Peter T.
AU - Sikkel, Markus
AU - Schobesberger, Sophie
AU - Diakonov, Ivan
AU - Novak, Pavel
AU - Castaldi, Alessandra
AU - Cattaneo, Paola
AU - Lyon, Alexander R.
AU - Lab, Max J.
AU - Gorelik, Julia
PY - 2016/1/5
Y1 - 2016/1/5
N2 - Arrhythmogenesis during heart failure is a major clinical problem. Regional electrical gradients produce arrhythmias, and cellular ionic transmembrane gradients are its originators. We investigated whether the nanoscale mechanosensitive properties of cardiomyocytes from failing hearts have a bearing upon the initiation of abnormal electrical activity. Hydrojets through a nanopipette indent specific locations on the sarcolemma and initiate intracellular calcium release in both healthy and heart failure cardiomyocytes, as well as in human failing cardiomyocytes. In healthy cells, calcium is locally confined, whereas in failing cardiomyocytes, calcium propagates. Heart failure progressively stiffens the membrane and displaces sub-sarcolemmal mitochondria. Colchicine in healthy cells mimics the failing condition by stiffening the cells, disrupting microtubules, shifting mitochondria, and causing calcium release. Uncoupling the mitochondrial proton gradient abolished calcium initiation in both failing and colchicine-treated cells. We propose the disruption of microtubule-dependent mitochondrial mechanosensor microdomains as a mechanism for abnormal calcium release in failing heart. Miragoli et al. show that failing heart cells have altered sensitivity to nanomechanical stimuli mediated by changes in the alignment of microtubules. The microtubule network disorganization leads to displacement of mitochondria and alterations in calcium release.
AB - Arrhythmogenesis during heart failure is a major clinical problem. Regional electrical gradients produce arrhythmias, and cellular ionic transmembrane gradients are its originators. We investigated whether the nanoscale mechanosensitive properties of cardiomyocytes from failing hearts have a bearing upon the initiation of abnormal electrical activity. Hydrojets through a nanopipette indent specific locations on the sarcolemma and initiate intracellular calcium release in both healthy and heart failure cardiomyocytes, as well as in human failing cardiomyocytes. In healthy cells, calcium is locally confined, whereas in failing cardiomyocytes, calcium propagates. Heart failure progressively stiffens the membrane and displaces sub-sarcolemmal mitochondria. Colchicine in healthy cells mimics the failing condition by stiffening the cells, disrupting microtubules, shifting mitochondria, and causing calcium release. Uncoupling the mitochondrial proton gradient abolished calcium initiation in both failing and colchicine-treated cells. We propose the disruption of microtubule-dependent mitochondrial mechanosensor microdomains as a mechanism for abnormal calcium release in failing heart. Miragoli et al. show that failing heart cells have altered sensitivity to nanomechanical stimuli mediated by changes in the alignment of microtubules. The microtubule network disorganization leads to displacement of mitochondria and alterations in calcium release.
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UR - http://www.scopus.com/inward/citedby.url?scp=84952979698&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2015.12.014
DO - 10.1016/j.celrep.2015.12.014
M3 - Article
AN - SCOPUS:84952979698
VL - 14
SP - 140
EP - 151
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 1
ER -