Microtubule-Dependent Mitochondria Alignment Regulates Calcium Release in Response to Nanomechanical Stimulus in Heart Myocytes

Michele Miragoli, Jose L. Sanchez-Alonso, Anamika Bhargava, Peter T. Wright, Markus Sikkel, Sophie Schobesberger, Ivan Diakonov, Pavel Novak, Alessandra Castaldi, Paola Cattaneo, Alexander R. Lyon, Max J. Lab, Julia Gorelik

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Arrhythmogenesis during heart failure is a major clinical problem. Regional electrical gradients produce arrhythmias, and cellular ionic transmembrane gradients are its originators. We investigated whether the nanoscale mechanosensitive properties of cardiomyocytes from failing hearts have a bearing upon the initiation of abnormal electrical activity. Hydrojets through a nanopipette indent specific locations on the sarcolemma and initiate intracellular calcium release in both healthy and heart failure cardiomyocytes, as well as in human failing cardiomyocytes. In healthy cells, calcium is locally confined, whereas in failing cardiomyocytes, calcium propagates. Heart failure progressively stiffens the membrane and displaces sub-sarcolemmal mitochondria. Colchicine in healthy cells mimics the failing condition by stiffening the cells, disrupting microtubules, shifting mitochondria, and causing calcium release. Uncoupling the mitochondrial proton gradient abolished calcium initiation in both failing and colchicine-treated cells. We propose the disruption of microtubule-dependent mitochondrial mechanosensor microdomains as a mechanism for abnormal calcium release in failing heart. Miragoli et al. show that failing heart cells have altered sensitivity to nanomechanical stimuli mediated by changes in the alignment of microtubules. The microtubule network disorganization leads to displacement of mitochondria and alterations in calcium release.

Original languageEnglish
Pages (from-to)140-151
Number of pages12
JournalCell Reports
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 5 2016

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Mitochondria
Microtubules
Muscle Cells
Calcium
Cardiac Myocytes
Heart Failure
Cells
Colchicine
Bearings (structural)
Sarcolemma
Protons
Cardiac Arrhythmias
Membranes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Microtubule-Dependent Mitochondria Alignment Regulates Calcium Release in Response to Nanomechanical Stimulus in Heart Myocytes. / Miragoli, Michele; Sanchez-Alonso, Jose L.; Bhargava, Anamika; Wright, Peter T.; Sikkel, Markus; Schobesberger, Sophie; Diakonov, Ivan; Novak, Pavel; Castaldi, Alessandra; Cattaneo, Paola; Lyon, Alexander R.; Lab, Max J.; Gorelik, Julia.

In: Cell Reports, Vol. 14, No. 1, 05.01.2016, p. 140-151.

Research output: Contribution to journalArticle

Miragoli, M, Sanchez-Alonso, JL, Bhargava, A, Wright, PT, Sikkel, M, Schobesberger, S, Diakonov, I, Novak, P, Castaldi, A, Cattaneo, P, Lyon, AR, Lab, MJ & Gorelik, J 2016, 'Microtubule-Dependent Mitochondria Alignment Regulates Calcium Release in Response to Nanomechanical Stimulus in Heart Myocytes', Cell Reports, vol. 14, no. 1, pp. 140-151. https://doi.org/10.1016/j.celrep.2015.12.014
Miragoli, Michele ; Sanchez-Alonso, Jose L. ; Bhargava, Anamika ; Wright, Peter T. ; Sikkel, Markus ; Schobesberger, Sophie ; Diakonov, Ivan ; Novak, Pavel ; Castaldi, Alessandra ; Cattaneo, Paola ; Lyon, Alexander R. ; Lab, Max J. ; Gorelik, Julia. / Microtubule-Dependent Mitochondria Alignment Regulates Calcium Release in Response to Nanomechanical Stimulus in Heart Myocytes. In: Cell Reports. 2016 ; Vol. 14, No. 1. pp. 140-151.
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