Microvescicles derived from mesenchymal stromal cells are not as effective as their cellular counterpart in the ability to modulate immune responses in vitro

Antonella Conforti, Marco Scarsella, Nadia Starc, Ezio Giorda, Simone Biagini, Alessandra Proia, Rita Carsetti, Franco Locatelli, Maria Ester Bernardo

Research output: Contribution to journalArticle

Abstract

Mesenchymal stromal cells (MSCs) are multipotent cells that possess broad immunomodulatory properties; the mechanisms underlying these properties have not been completely clarified. Aim of this study was to compare in vitro immunomodulatory effects of MSCs with those of microvesicles (MVs) released in supernatants from the same MSCs. MSCs were generated from bone marrow of 12 healthy donors (HDs) and MVs were isolated from their supernatant by serial ultracentrifugation according to two different procedures. Both MSCs and MVs were characterized by flow cytometry and incubated in vitro with peripheral blood mononuclear cells (PBMCs) of 12 HDs after stimulation with PHA and CpG. Growth factors and cytokines were quantified by ELISA. MVs were identified as 0.1-1μm particles positive for CMFDA, CD107, and CD13. MSCs were significantly more capable to inhibit in vitro PHA-induced T-cell proliferation as compared with the corresponding MVs (P

Original languageEnglish
Pages (from-to)2591-2599
Number of pages9
JournalStem Cells and Development
Volume23
Issue number21
DOIs
Publication statusPublished - Nov 1 2014

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Mesenchymal Stromal Cells
Ultracentrifugation
In Vitro Techniques
Blood Cells
Intercellular Signaling Peptides and Proteins
Flow Cytometry
Bone Marrow
Enzyme-Linked Immunosorbent Assay
Cell Proliferation
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology
  • Medicine(all)

Cite this

Microvescicles derived from mesenchymal stromal cells are not as effective as their cellular counterpart in the ability to modulate immune responses in vitro. / Conforti, Antonella; Scarsella, Marco; Starc, Nadia; Giorda, Ezio; Biagini, Simone; Proia, Alessandra; Carsetti, Rita; Locatelli, Franco; Bernardo, Maria Ester.

In: Stem Cells and Development, Vol. 23, No. 21, 01.11.2014, p. 2591-2599.

Research output: Contribution to journalArticle

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