Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells

Valentina Bollati, Laura Angelici, Giovanna Rizzo, Laura Pergoli, Federica Rota, Mirjam Hoxha, Francesco Nordio, Matteo Bonzini, Letizia Tarantini, Laura Cantone, Angela C. Pesatori, Pietro Apostoli, Andrea A. Baccarelli, Pier Alberto Bertazzi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Cardiovascular disease risk has been consistently linked with particulate matter (PM) exposure. Cell-derived microvesicles (MVs) are released into plasma and transfer microRNAs (miRNAs) between tissues. MVs can be produced by the respiratory system in response to proinflammatory triggers, enter the circulatory system and remotely modify gene expression in cardiovascular tissues. However, whether PM affects MV signaling has never been investigated. In this study, we evaluated expression of microRNAs contained within plasma MVs upon PM exposure both in vivo and in vitro. In the in vivo study, we isolated plasma MVs from healthy steel plant workers before and after workplace PM exposure. We measured the expression of 88 MV-associated miRNAs by real-time polymerase chain reaction. To assess a possible source of the MV miRNAs identified in vivo, we measured their miRNA expression in PM-treated A549 pulmonary cell lines in vitro. MiRNA profiling of plasma MVs showed 5.62- and 13.95-fold increased expression of miR-128 and miR-302c, respectively, after 3 days of workplace PM exposure (P

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalJournal of Applied Toxicology
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Particulate Matter
MicroRNAs
Plasmas
Workplace
Tissue
Respiratory system
Iron and steel plants
Steel
Polymerase chain reaction
Cardiovascular System
Gene expression
Respiratory System
A549 Cells
Real-Time Polymerase Chain Reaction
Cardiovascular Diseases
Cells
Gene Expression
Lung

Keywords

  • A549 cells
  • MicroRNAs
  • Microvesicles
  • Particulate matter
  • Steel plant workers

ASJC Scopus subject areas

  • Toxicology

Cite this

Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells. / Bollati, Valentina; Angelici, Laura; Rizzo, Giovanna; Pergoli, Laura; Rota, Federica; Hoxha, Mirjam; Nordio, Francesco; Bonzini, Matteo; Tarantini, Letizia; Cantone, Laura; Pesatori, Angela C.; Apostoli, Pietro; Baccarelli, Andrea A.; Bertazzi, Pier Alberto.

In: Journal of Applied Toxicology, Vol. 35, No. 1, 01.01.2015, p. 59-67.

Research output: Contribution to journalArticle

Bollati, V, Angelici, L, Rizzo, G, Pergoli, L, Rota, F, Hoxha, M, Nordio, F, Bonzini, M, Tarantini, L, Cantone, L, Pesatori, AC, Apostoli, P, Baccarelli, AA & Bertazzi, PA 2015, 'Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells', Journal of Applied Toxicology, vol. 35, no. 1, pp. 59-67. https://doi.org/10.1002/jat.2987
Bollati, Valentina ; Angelici, Laura ; Rizzo, Giovanna ; Pergoli, Laura ; Rota, Federica ; Hoxha, Mirjam ; Nordio, Francesco ; Bonzini, Matteo ; Tarantini, Letizia ; Cantone, Laura ; Pesatori, Angela C. ; Apostoli, Pietro ; Baccarelli, Andrea A. ; Bertazzi, Pier Alberto. / Microvesicle-associated microRNA expression is altered upon particulate matter exposure in healthy workers and in A549 cells. In: Journal of Applied Toxicology. 2015 ; Vol. 35, No. 1. pp. 59-67.
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