Migratory response of human NK cells to monococyte-chemotactic proteins

P. Allavena, G. Bianchi, P. Giardina, N. Polentarutti, D. Zhou, M. Introna, S. Sozzani, A. Mantovani

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

NK cells are present mostly in blood and spleen but under certain pathological and physiological conditions rapidly accumulate at extrahematic sites. The present study investigates the responsiveness of NK cells to C-C chemokines and the mechanisms of emigration from the bloodstream. MCP-1 induced migration across polycarbonate filters of IL-2-activated NK cells, whereas it was a weak attractant for unstimulated cells. The related chemokines MCP-2 and MCP-3 were also active. IL-2-activated NK cells showed specific binding sites for labeled MCP-1, and cell migration was inhibited by both cholera and Bordetella pertussis toxins. In agreement with functional assays the expression of mRNA specific for MCP-1 receptors was detectable only in IL-2-activated NK cells. The ability of NK cells to respond to MCP-1 and related chemokines may be one important determinant of NK cell emigration and recruitment in tissues.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalMethods in Enzymology
Volume10
Issue number1
DOIs
Publication statusPublished - Aug 1996

Fingerprint

Natural Killer Cells
Interleukin-2
polycarbonate
Chemokines
CCR2 Receptors
CC Chemokines
Proteins
Pertussis Toxin
Emigration and Immigration
Assays
Blood
Binding Sites
Tissue
Messenger RNA
Bordetella pertussis
Cholera
Cell Movement
Spleen

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Migratory response of human NK cells to monococyte-chemotactic proteins. / Allavena, P.; Bianchi, G.; Giardina, P.; Polentarutti, N.; Zhou, D.; Introna, M.; Sozzani, S.; Mantovani, A.

In: Methods in Enzymology, Vol. 10, No. 1, 08.1996, p. 145-149.

Research output: Contribution to journalArticle

@article{20fb7c7d5781470dbea4e1dc61cf4892,
title = "Migratory response of human NK cells to monococyte-chemotactic proteins",
abstract = "NK cells are present mostly in blood and spleen but under certain pathological and physiological conditions rapidly accumulate at extrahematic sites. The present study investigates the responsiveness of NK cells to C-C chemokines and the mechanisms of emigration from the bloodstream. MCP-1 induced migration across polycarbonate filters of IL-2-activated NK cells, whereas it was a weak attractant for unstimulated cells. The related chemokines MCP-2 and MCP-3 were also active. IL-2-activated NK cells showed specific binding sites for labeled MCP-1, and cell migration was inhibited by both cholera and Bordetella pertussis toxins. In agreement with functional assays the expression of mRNA specific for MCP-1 receptors was detectable only in IL-2-activated NK cells. The ability of NK cells to respond to MCP-1 and related chemokines may be one important determinant of NK cell emigration and recruitment in tissues.",
author = "P. Allavena and G. Bianchi and P. Giardina and N. Polentarutti and D. Zhou and M. Introna and S. Sozzani and A. Mantovani",
year = "1996",
month = "8",
doi = "10.1006/meth.1996.0088",
language = "English",
volume = "10",
pages = "145--149",
journal = "ImmunoMethods",
issn = "1046-2023",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Migratory response of human NK cells to monococyte-chemotactic proteins

AU - Allavena, P.

AU - Bianchi, G.

AU - Giardina, P.

AU - Polentarutti, N.

AU - Zhou, D.

AU - Introna, M.

AU - Sozzani, S.

AU - Mantovani, A.

PY - 1996/8

Y1 - 1996/8

N2 - NK cells are present mostly in blood and spleen but under certain pathological and physiological conditions rapidly accumulate at extrahematic sites. The present study investigates the responsiveness of NK cells to C-C chemokines and the mechanisms of emigration from the bloodstream. MCP-1 induced migration across polycarbonate filters of IL-2-activated NK cells, whereas it was a weak attractant for unstimulated cells. The related chemokines MCP-2 and MCP-3 were also active. IL-2-activated NK cells showed specific binding sites for labeled MCP-1, and cell migration was inhibited by both cholera and Bordetella pertussis toxins. In agreement with functional assays the expression of mRNA specific for MCP-1 receptors was detectable only in IL-2-activated NK cells. The ability of NK cells to respond to MCP-1 and related chemokines may be one important determinant of NK cell emigration and recruitment in tissues.

AB - NK cells are present mostly in blood and spleen but under certain pathological and physiological conditions rapidly accumulate at extrahematic sites. The present study investigates the responsiveness of NK cells to C-C chemokines and the mechanisms of emigration from the bloodstream. MCP-1 induced migration across polycarbonate filters of IL-2-activated NK cells, whereas it was a weak attractant for unstimulated cells. The related chemokines MCP-2 and MCP-3 were also active. IL-2-activated NK cells showed specific binding sites for labeled MCP-1, and cell migration was inhibited by both cholera and Bordetella pertussis toxins. In agreement with functional assays the expression of mRNA specific for MCP-1 receptors was detectable only in IL-2-activated NK cells. The ability of NK cells to respond to MCP-1 and related chemokines may be one important determinant of NK cell emigration and recruitment in tissues.

UR - http://www.scopus.com/inward/record.url?scp=0030221127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030221127&partnerID=8YFLogxK

U2 - 10.1006/meth.1996.0088

DO - 10.1006/meth.1996.0088

M3 - Article

VL - 10

SP - 145

EP - 149

JO - ImmunoMethods

JF - ImmunoMethods

SN - 1046-2023

IS - 1

ER -