Abstract
A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of 4,2 × 10 6 cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.
| Original language | English |
|---|---|
| Article number | 326813 |
| Journal | Stem Cells International |
| DOIs | |
| Publication status | Published - 2012 |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering : An in vitro study. / Marmotti, A.; Mattia, S.; Bruzzone, M.; Buttiglieri, S.; Risso, A.; Bonasia, D. E.; Blonna, D.; Castoldi, F.; Rossi, R.; Zanini, C.; Ercole, E.; Defabiani, E.; Tarella, C.; Peretti, G. M.
In: Stem Cells International, 2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering
T2 - An in vitro study
AU - Marmotti, A.
AU - Mattia, S.
AU - Bruzzone, M.
AU - Buttiglieri, S.
AU - Risso, A.
AU - Bonasia, D. E.
AU - Blonna, D.
AU - Castoldi, F.
AU - Rossi, R.
AU - Zanini, C.
AU - Ercole, E.
AU - Defabiani, E.
AU - Tarella, C.
AU - Peretti, G. M.
PY - 2012
Y1 - 2012
N2 - A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of 4,2 × 10 6 cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.
AB - A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of 4,2 × 10 6 cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.
UR - http://www.scopus.com/inward/record.url?scp=84859741801&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84859741801&partnerID=8YFLogxK
U2 - 10.1155/2012/326813
DO - 10.1155/2012/326813
M3 - Article
C2 - 22550503
AN - SCOPUS:84859741801
JO - Stem Cells International
JF - Stem Cells International
SN - 1687-966X
M1 - 326813
ER -