Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: An in vitro study

A. Marmotti, S. Mattia, M. Bruzzone, S. Buttiglieri, A. Risso, D. E. Bonasia, D. Blonna, F. Castoldi, R. Rossi, C. Zanini, E. Ercole, E. Defabiani, C. Tarella, G. M. Peretti

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of 4,2 × 10 6 cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.

Original languageEnglish
Article number326813
JournalStem Cells International
DOIs
Publication statusPublished - 2012

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Umbilical Cord
Tissue Engineering
Orthopedics
Telomere
Mesenchymal Stromal Cells
Cartilage
Blood Vessels
Regeneration
Digestion
Bone Marrow
Fats
In Vitro Techniques
Bone and Bones
Muscles
Population

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering : An in vitro study. / Marmotti, A.; Mattia, S.; Bruzzone, M.; Buttiglieri, S.; Risso, A.; Bonasia, D. E.; Blonna, D.; Castoldi, F.; Rossi, R.; Zanini, C.; Ercole, E.; Defabiani, E.; Tarella, C.; Peretti, G. M.

In: Stem Cells International, 2012.

Research output: Contribution to journalArticle

Marmotti, A, Mattia, S, Bruzzone, M, Buttiglieri, S, Risso, A, Bonasia, DE, Blonna, D, Castoldi, F, Rossi, R, Zanini, C, Ercole, E, Defabiani, E, Tarella, C & Peretti, GM 2012, 'Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: An in vitro study', Stem Cells International. https://doi.org/10.1155/2012/326813
Marmotti, A. ; Mattia, S. ; Bruzzone, M. ; Buttiglieri, S. ; Risso, A. ; Bonasia, D. E. ; Blonna, D. ; Castoldi, F. ; Rossi, R. ; Zanini, C. ; Ercole, E. ; Defabiani, E. ; Tarella, C. ; Peretti, G. M. / Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering : An in vitro study. In: Stem Cells International. 2012.
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