Mineralization behavior with mesenchymal stromal cells in a biomimetic hyaluronic acid-based scaffold

Cristina Manferdini, Vincenzo Guarino, Nicoletta Zini, Maria Grazia Raucci, Andrea Ferrari, Francesco Grassi, Elena Gabusi, Stefano Squarzoni, Andrea Facchini, Luigi Ambrosio, Gina Lisignoli

Research output: Contribution to journalArticlepeer-review


A biomimetic hyaluronic acid (HA)-based polymer scaffold was analysed in vitro for its characteristics and potential to support mineralization as carrier-vehicle. Biomimetic apatite crystal nucleation on the scaffold surface was obtained by a fine control of the pH level that increased ionic solubility thus controlling apatite formation kinetic. Different concentrations of human mesenchymal stromal cells (h-MSCs) were seeded on the scaffold, osteogenesis was induced in the presence or absence of fibroblast growth factor -2 and mineralization was analysed at different time points. We found that only at the highest h-MSCs concentration tested, the cells were uniformly distributed inside and outside the scaffold and proliferation started to decrease from day 7. Electron microscopy analysis evidenced that h-MSCs produced extracellular matrix but did not establish a direct contact with the scaffold. We found mineralized calcium-positive areas mainly present along the backbone of the scaffold starting from day 21 and increasing at day 35. FGF-2 treatment did not accelerate or increase mineralization. Non-biomimetic HA-based control scaffold showed immature mineralized areas only at day 35. Our data demonstrate that the biomimetic treatment of an HA-based scaffold promotes a faster mineralization process suggesting its possible use in clinics as a support for improving bone repair.

Original languageEnglish
Pages (from-to)3986-3996
Number of pages11
Issue number14
Publication statusPublished - May 2010


  • Biomimetic material
  • Fibroblast growth factor
  • Hyaluronic acid
  • Osteogenesis
  • SBF

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics


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