Mini-FLOTAC and Kato-Katz: Helminth eggs watching on the shore of lake Victoria

Beatrice Barda, Henry Zepherine, Laura Rinaldi, Giuseppe Cringoli, Roberto Burioni, Massimo Clementi, Marco Albonico

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Background: One of the challenges for monitoring helminth control programmes based on preventive chemotherapy is the lack of a copro-parasitological gold-standard method that combines good sensitivity with quantitative performance, low cost, and easy-to-learn technique. The aim of our study was to evaluate and compare, the WHO recommended quantitative diagnostic technique (Kato-Katz) and the Mini-FLOTAC. Methods. Mini-FLOTAC is an innovative method based on floatation of helminths eggs with two different solutions (FS2 and FS7) using a close system (Fill-FLOTAC) with 5% fixative. Kato-Katz was performed following WHO recommendation. The study was carried out in a rural part of Tanzania, close to Lake Victoria, where the laboratory facilities are fairly scarce, and the basic technique used in the local laboratory (direct smear) was taken as reference standard. Results: 201 children were screened for intestinal helminths and 91% of them were found to be positive. The agreement among the three techniques was calculated with k Cohen coefficient and was fairly good (k = 0.4), although the Mini-FLOTAC results were more sensitive for hookworm (98%) with FS2, and for S.mansoni (90%) with FS7 followed by Kato-Katz (91% and 60% respectively) and direct smear (30% and 10% respectively). A good agreement was found between Mini-FLOTAC and Kato-Katz (k = 0.81) with FS7 (k = 0.76) for hookworm diagnosis and a fairly good one for S.mansoni diagnosis (k = 0.5). For both infections we had a poor agreement between the two quantitative techniques and the direct smear (k

Original languageEnglish
Article number220
JournalParasites and Vectors
Issue number1
Publication statusPublished - 2013


  • Kato-Katz method
  • Lake Victoria Tanzania
  • Mini-FLOTAC technique
  • Soil-transmitted helminths

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases


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