Mini-FLOTAC, Kato-Katz and McMaster: Three methods, one goal; Highlights from north Argentina

Beatrice Barda, Pamela Cajal, Eliana Villagran, Ruben Cimino, Marisa Juarez, Alejandro Krolewiecki, Laura Rinaldi, Giuseppe Cringoli, Roberto Burioni, Marco Albonico

Research output: Contribution to journalArticle

Abstract

Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individual and community levels in resource-limited settings. The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster and Mini-FLOTAC methids. The study was carried out in Oran, Northern Argentina. Methods. 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminth infections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotation solutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methods was calculated both when processing single and multiple samples. Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; the most prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of less prevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2 was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was more sensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A. lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for egg counts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111 with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected the highest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences were not statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample, Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC, the mean time (min/sample) decreased significantly when processing multiple samples. Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz and McMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification. Egg counts differences although relevant, did not reach statistical significance.

Original languageEnglish
Article number271
JournalParasites and Vectors
Volume7
Issue number1
DOIs
Publication statusPublished - Jun 14 2014

Fingerprint

Helminths
Argentina
Hymenolepis nana
Ascaris lumbricoides
Feces
Eggs
Ovum
Infection
Enterobius
Zinc Sulfate
Trichuris
Ancylostomatoidea
Reading

Keywords

  • Diagnostic techniques
  • Kato-Katz thick smear
  • McMaster method
  • Mini-FLOTAC technique
  • Soil-transmitted helminths

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Medicine(all)

Cite this

Barda, B., Cajal, P., Villagran, E., Cimino, R., Juarez, M., Krolewiecki, A., ... Albonico, M. (2014). Mini-FLOTAC, Kato-Katz and McMaster: Three methods, one goal; Highlights from north Argentina. Parasites and Vectors, 7(1), [271]. https://doi.org/10.1186/1756-3305-7-271

Mini-FLOTAC, Kato-Katz and McMaster : Three methods, one goal; Highlights from north Argentina. / Barda, Beatrice; Cajal, Pamela; Villagran, Eliana; Cimino, Ruben; Juarez, Marisa; Krolewiecki, Alejandro; Rinaldi, Laura; Cringoli, Giuseppe; Burioni, Roberto; Albonico, Marco.

In: Parasites and Vectors, Vol. 7, No. 1, 271, 14.06.2014.

Research output: Contribution to journalArticle

Barda, B, Cajal, P, Villagran, E, Cimino, R, Juarez, M, Krolewiecki, A, Rinaldi, L, Cringoli, G, Burioni, R & Albonico, M 2014, 'Mini-FLOTAC, Kato-Katz and McMaster: Three methods, one goal; Highlights from north Argentina', Parasites and Vectors, vol. 7, no. 1, 271. https://doi.org/10.1186/1756-3305-7-271
Barda, Beatrice ; Cajal, Pamela ; Villagran, Eliana ; Cimino, Ruben ; Juarez, Marisa ; Krolewiecki, Alejandro ; Rinaldi, Laura ; Cringoli, Giuseppe ; Burioni, Roberto ; Albonico, Marco. / Mini-FLOTAC, Kato-Katz and McMaster : Three methods, one goal; Highlights from north Argentina. In: Parasites and Vectors. 2014 ; Vol. 7, No. 1.
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abstract = "Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individual and community levels in resource-limited settings. The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster and Mini-FLOTAC methids. The study was carried out in Oran, Northern Argentina. Methods. 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminth infections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotation solutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methods was calculated both when processing single and multiple samples. Results: Out of 193 schoolchildren examined, 40{\%} were positive for any helminth infection by any method; the most prevalent was Hymenolepis nana (23{\%}) followed by Ascaris lumbricoides (17{\%}) and a third group of less prevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11{\%} all together). Mini-FLOTAC FS2 was more sensitive than FS7 for H. nana (93{\%} vs 78{\%}) and for other helminths (85{\%} vs 80{\%}), whereas FS7 was more sensitive for A. lumbricoides (87{\%} vs 61{\%}). Kato-Katz method was more sensitive than McMaster method for A. lumbricoides (84{\%} vs 48{\%}) and for other helminths (48{\%} vs 43{\%}) except for H. nana (49{\%} vs 61{\%}). As for egg counts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111 with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected the highest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences were not statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample, Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC, the mean time (min/sample) decreased significantly when processing multiple samples. Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz and McMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification. Egg counts differences although relevant, did not reach statistical significance.",
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T1 - Mini-FLOTAC, Kato-Katz and McMaster

T2 - Three methods, one goal; Highlights from north Argentina

AU - Barda, Beatrice

AU - Cajal, Pamela

AU - Villagran, Eliana

AU - Cimino, Ruben

AU - Juarez, Marisa

AU - Krolewiecki, Alejandro

AU - Rinaldi, Laura

AU - Cringoli, Giuseppe

AU - Burioni, Roberto

AU - Albonico, Marco

PY - 2014/6/14

Y1 - 2014/6/14

N2 - Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individual and community levels in resource-limited settings. The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster and Mini-FLOTAC methids. The study was carried out in Oran, Northern Argentina. Methods. 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminth infections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotation solutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methods was calculated both when processing single and multiple samples. Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; the most prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of less prevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2 was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was more sensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A. lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for egg counts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111 with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected the highest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences were not statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample, Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC, the mean time (min/sample) decreased significantly when processing multiple samples. Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz and McMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification. Egg counts differences although relevant, did not reach statistical significance.

AB - Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individual and community levels in resource-limited settings. The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster and Mini-FLOTAC methids. The study was carried out in Oran, Northern Argentina. Methods. 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminth infections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotation solutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methods was calculated both when processing single and multiple samples. Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; the most prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of less prevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2 was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was more sensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A. lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for egg counts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111 with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected the highest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences were not statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample, Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC, the mean time (min/sample) decreased significantly when processing multiple samples. Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz and McMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification. Egg counts differences although relevant, did not reach statistical significance.

KW - Diagnostic techniques

KW - Kato-Katz thick smear

KW - McMaster method

KW - Mini-FLOTAC technique

KW - Soil-transmitted helminths

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