Minimal residual disease status in transplanted chronic myelogenous leukemia patients: Low incidence of polymerase chain reaction positive cases among 48 long disease-free subjects who received unmanipulated allogeneic bone marrow transplants

Angelo Guerrasio, Giovanni Martinelli, Giuseppe Saglio, Claudia Rosso, Alfonso Zaccaria, Gianantonio Rosti, Nicoletta Testoni, Achille Ambrosetti, Teo Izzi, Mario Sessarego, Francesco Frassoni, Paolo Gasparini, Andrea Chiamenti, Paolo Di Bartolomeo, Pier Franco Pignatti

Research output: Contribution to journalArticle

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Abstract

Forty-eight long-term disease-free chronic myelogenous leukemia (CML) patients, who had received unmanipulated allogeneic bone marrow transplants (BMT) for eradication of the Philadelphia (Ph1)-positive clone were studied by polymerase chain reaction (PCR), using a very sensitive PCR procedure and very stringent criteria for preventing and revealing contamination. Nine patients (18%) were positive at the first PCR examination, but only one patient remained PCR positive four years after. However, a second PCR analysis performed on new bone marrow samples obtained at a median interval of 14 months (range 6-16) after the first specimen collection from six of nine originally positive cases, and from 16 of 39 originally negative cases, showed that only one of the six positive cases remained positive, whereas negativity was confirmed in all the originally negative patients. These data are evidence that the Ph1-positive clone is apparently completely eradicated in the majority of CML patients who survive disease-free long-term after an unmanipulated allogeneic BMT and that only sporadic cases remain PCR-positive four years post-BMT. The data also show that at least two sequential bone marrow samples for each patient must be analyzed before drawing conclusions regarding the stable persistence of BCR/ABL transcripts and the minimal residual disease status.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalLeukemia
Volume6
Issue number6
Publication statusPublished - Jun 1992

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Residual Neoplasm
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Bone Marrow
Transplants
Polymerase Chain Reaction
Incidence
Clone Cells
Specimen Handling

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Minimal residual disease status in transplanted chronic myelogenous leukemia patients : Low incidence of polymerase chain reaction positive cases among 48 long disease-free subjects who received unmanipulated allogeneic bone marrow transplants. / Guerrasio, Angelo; Martinelli, Giovanni; Saglio, Giuseppe; Rosso, Claudia; Zaccaria, Alfonso; Rosti, Gianantonio; Testoni, Nicoletta; Ambrosetti, Achille; Izzi, Teo; Sessarego, Mario; Frassoni, Francesco; Gasparini, Paolo; Chiamenti, Andrea; Di Bartolomeo, Paolo; Pignatti, Pier Franco.

In: Leukemia, Vol. 6, No. 6, 06.1992, p. 507-512.

Research output: Contribution to journalArticle

Guerrasio, A, Martinelli, G, Saglio, G, Rosso, C, Zaccaria, A, Rosti, G, Testoni, N, Ambrosetti, A, Izzi, T, Sessarego, M, Frassoni, F, Gasparini, P, Chiamenti, A, Di Bartolomeo, P & Pignatti, PF 1992, 'Minimal residual disease status in transplanted chronic myelogenous leukemia patients: Low incidence of polymerase chain reaction positive cases among 48 long disease-free subjects who received unmanipulated allogeneic bone marrow transplants', Leukemia, vol. 6, no. 6, pp. 507-512.
Guerrasio, Angelo ; Martinelli, Giovanni ; Saglio, Giuseppe ; Rosso, Claudia ; Zaccaria, Alfonso ; Rosti, Gianantonio ; Testoni, Nicoletta ; Ambrosetti, Achille ; Izzi, Teo ; Sessarego, Mario ; Frassoni, Francesco ; Gasparini, Paolo ; Chiamenti, Andrea ; Di Bartolomeo, Paolo ; Pignatti, Pier Franco. / Minimal residual disease status in transplanted chronic myelogenous leukemia patients : Low incidence of polymerase chain reaction positive cases among 48 long disease-free subjects who received unmanipulated allogeneic bone marrow transplants. In: Leukemia. 1992 ; Vol. 6, No. 6. pp. 507-512.
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abstract = "Forty-eight long-term disease-free chronic myelogenous leukemia (CML) patients, who had received unmanipulated allogeneic bone marrow transplants (BMT) for eradication of the Philadelphia (Ph1)-positive clone were studied by polymerase chain reaction (PCR), using a very sensitive PCR procedure and very stringent criteria for preventing and revealing contamination. Nine patients (18{\%}) were positive at the first PCR examination, but only one patient remained PCR positive four years after. However, a second PCR analysis performed on new bone marrow samples obtained at a median interval of 14 months (range 6-16) after the first specimen collection from six of nine originally positive cases, and from 16 of 39 originally negative cases, showed that only one of the six positive cases remained positive, whereas negativity was confirmed in all the originally negative patients. These data are evidence that the Ph1-positive clone is apparently completely eradicated in the majority of CML patients who survive disease-free long-term after an unmanipulated allogeneic BMT and that only sporadic cases remain PCR-positive four years post-BMT. The data also show that at least two sequential bone marrow samples for each patient must be analyzed before drawing conclusions regarding the stable persistence of BCR/ABL transcripts and the minimal residual disease status.",
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