miR-135b suppresses tumorigenesis in glioblastoma stem-like cells impairing proliferation, migration and self-renewal

Valentina Lulli, Mariachiara Buccarelli, Maurizio Martini, Michele Signore, Mauro Biffoni, Stefano Giannetti, Liliana Morgante, Giovanna Marziali, Ramona Ilari, Alfredo Pagliuca, Luigi Maria Larocca, Ruggero de Maria, Roberto Pallini, Lucia Ricci-Vitiani

Research output: Contribution to journalArticle

Abstract

Glioblastoma multiforme (GBM) is the most common and fatal malignant adult primary brain tumor. Currently, the overall prognosis for GBM patients remains poor despite advances in neurosurgery and adjuvant treatments. MicroRNAs (miRNAs) contribute to the pathogenesis of various types of tumor, including GBM. In this study we analyzed the expression of a panel of miRNAs, which are known to be differentially expressed by the brain and GBM tumor, in a collection of patient-derived GBM stem-like cells (GSCs). Notably, the average expression level of miR-135b, was the most downregulated compared to its normal counterpart, suggesting a potential role as anti-oncogene. Restoration of miR-135b in GSCs significantly decreased proliferation, migration and clonogenic abilities. More importantly, miR-135b restoration was able to significantly reduce brain infiltration in mouse models of GBM obtained by intracerebral injection of GSC lines. We identified ADAM12 and confirmed SMAD5 and GSK3β as miR-135b targets and potential mediators of its effects. The whole transcriptome analysis ascertained that the expression of miR-135b downmodulated additional genes driving key pathways in GBM survival and infiltration capabilities. Our results identify a critical role of miR-135b in the regulation of GBM development, suggesting that miR-135b might act as a tumor-suppressor factor and thus providing a potential candidate for the treatment of GBM patients.

Original languageEnglish
Pages (from-to)37241-37256
Number of pages16
JournalOncotarget
Volume6
Issue number35
DOIs
Publication statusPublished - 2015

Fingerprint

Glioblastoma
Carcinogenesis
Stem Cells
Cell Proliferation
MicroRNAs
Neoplasms
Brain
Neurosurgery
Gene Expression Profiling
Tumor Suppressor Genes
Brain Neoplasms
Down-Regulation
Cell Line
Injections
Survival
Therapeutics

Keywords

  • ADAM12
  • Glioblastoma
  • Glioblastoma stem cells
  • GSK3β
  • miRNAs
  • SMAD5

ASJC Scopus subject areas

  • Oncology

Cite this

Lulli, V., Buccarelli, M., Martini, M., Signore, M., Biffoni, M., Giannetti, S., ... Ricci-Vitiani, L. (2015). miR-135b suppresses tumorigenesis in glioblastoma stem-like cells impairing proliferation, migration and self-renewal. Oncotarget, 6(35), 37241-37256. https://doi.org/10.18632/oncotarget.5925

miR-135b suppresses tumorigenesis in glioblastoma stem-like cells impairing proliferation, migration and self-renewal. / Lulli, Valentina; Buccarelli, Mariachiara; Martini, Maurizio; Signore, Michele; Biffoni, Mauro; Giannetti, Stefano; Morgante, Liliana; Marziali, Giovanna; Ilari, Ramona; Pagliuca, Alfredo; Larocca, Luigi Maria; de Maria, Ruggero; Pallini, Roberto; Ricci-Vitiani, Lucia.

In: Oncotarget, Vol. 6, No. 35, 2015, p. 37241-37256.

Research output: Contribution to journalArticle

Lulli, V, Buccarelli, M, Martini, M, Signore, M, Biffoni, M, Giannetti, S, Morgante, L, Marziali, G, Ilari, R, Pagliuca, A, Larocca, LM, de Maria, R, Pallini, R & Ricci-Vitiani, L 2015, 'miR-135b suppresses tumorigenesis in glioblastoma stem-like cells impairing proliferation, migration and self-renewal', Oncotarget, vol. 6, no. 35, pp. 37241-37256. https://doi.org/10.18632/oncotarget.5925
Lulli, Valentina ; Buccarelli, Mariachiara ; Martini, Maurizio ; Signore, Michele ; Biffoni, Mauro ; Giannetti, Stefano ; Morgante, Liliana ; Marziali, Giovanna ; Ilari, Ramona ; Pagliuca, Alfredo ; Larocca, Luigi Maria ; de Maria, Ruggero ; Pallini, Roberto ; Ricci-Vitiani, Lucia. / miR-135b suppresses tumorigenesis in glioblastoma stem-like cells impairing proliferation, migration and self-renewal. In: Oncotarget. 2015 ; Vol. 6, No. 35. pp. 37241-37256.
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