TY - JOUR
T1 - miR-143 expression profiles in urinary bladder cancer
T2 - correlation with clinical and epidemiological parameters
AU - Boubaker, Nouha Setti
AU - Spagnuolo, Manuela
AU - Trabelsi, Nesrine
AU - Said, Rahma
AU - Gurtner, Aymone
AU - Regazzo, Giulia
AU - Ayed, Haroun
AU - Blel, Ahlem
AU - Karray, Omar
AU - Saadi, Ahmed
AU - Rammeh, Soumaya
AU - Chebil, Mohamed
AU - Rizzo, Maria Giulia
AU - Piaggio, Giulia
AU - Ouerhani, Slah
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Hsa-mir-143 and hsa-let-7c have been reported to be deregulated in multiple neoplasms. The main purpose of this study was to investigate the expression of these miRNAs in bladder cancer (BCa) and to analyze the association between their expression profiles and clinical and epidemiological parameters. Ninety BCa specimens were included. Expression patterns of miR-143 and let-7c were assessed by qRT-PCR using Taqman specific probes. Validated and predicted targets of these miRNA’s were identified using CSmiRTar and DAVID tools, respectively. miR-143 was downregulated in tumors compared to controls (mean fold-change (FC) = 0.076). Its expression was significantly higher in MIBC compared to NMIBC (p = 0,001). Its value as a potential biomarker discriminating non invasive tumors from the invasive ones was confirmed by ROC curve (AUC = 0.768; p = 0.0001). Also, this down-regulation positively correlates with frequency of tobacco use (p = 0,04) and chronic alcohol consumption (p = 0,04). Let-7c was overexpressed in BCa samples (mean (FC = 9.92) compared to non tumoral ones but was not associated to clinical and epidemiological parameters. A comprehensive overview of miR-143 targets and pathways implicated in BCa initiation, diagnosis or prognosis using bioinformatical analysis, was conducted. While both deregulated miRNAs may contribute to urothelial tumorigenesis, the deregulation of miR-143 was significantly correlated to epidemiological and clinical parameters.
AB - Hsa-mir-143 and hsa-let-7c have been reported to be deregulated in multiple neoplasms. The main purpose of this study was to investigate the expression of these miRNAs in bladder cancer (BCa) and to analyze the association between their expression profiles and clinical and epidemiological parameters. Ninety BCa specimens were included. Expression patterns of miR-143 and let-7c were assessed by qRT-PCR using Taqman specific probes. Validated and predicted targets of these miRNA’s were identified using CSmiRTar and DAVID tools, respectively. miR-143 was downregulated in tumors compared to controls (mean fold-change (FC) = 0.076). Its expression was significantly higher in MIBC compared to NMIBC (p = 0,001). Its value as a potential biomarker discriminating non invasive tumors from the invasive ones was confirmed by ROC curve (AUC = 0.768; p = 0.0001). Also, this down-regulation positively correlates with frequency of tobacco use (p = 0,04) and chronic alcohol consumption (p = 0,04). Let-7c was overexpressed in BCa samples (mean (FC = 9.92) compared to non tumoral ones but was not associated to clinical and epidemiological parameters. A comprehensive overview of miR-143 targets and pathways implicated in BCa initiation, diagnosis or prognosis using bioinformatical analysis, was conducted. While both deregulated miRNAs may contribute to urothelial tumorigenesis, the deregulation of miR-143 was significantly correlated to epidemiological and clinical parameters.
KW - biomarkers
KW - miRNA
KW - neoplasms
KW - pathways
KW - prognosis
KW - Urinary bladder
UR - http://www.scopus.com/inward/record.url?scp=85076880234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076880234&partnerID=8YFLogxK
U2 - 10.1007/s11033-019-05228-1
DO - 10.1007/s11033-019-05228-1
M3 - Article
AN - SCOPUS:85076880234
JO - Molecular Biology Reports
JF - Molecular Biology Reports
SN - 0301-4851
ER -