MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer

Resham Bhattacharya, Milena Nicoloso, Rochelle Arvizo, Enfeng Wang, Angelica Cortez, Simona Rossi, George A. Calin, Priyabrata Mukherjee

Research output: Contribution to journalArticle

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Abstract

Oncogenic activation of Bmi-1 is found in a wide variety of epithelial malignancies including ovarian cancer, yet a specific mechanism for overexpression of Bmi-1 has not been determined. Thus, realizing the immense pathologic significance of Bmi-1 in cancer, we wanted to investigate if microRNA (miRNA) aberrations played a role in the regulation of Bmi-1 in ovarian cancer. In this report, we identify two miRNAs, miR-15a and miR-16, that are underexpressed in ovarian cell lines and in primary ovarian tissues. We show that these miRNAs directly target the Bmi-1 3′ untranslated region and significantly correlate with Bmi-1 protein levels in ovarian cancer patients and cell lines. Furthermore, Bmi-1 protein levels are downregulated in response to miR-15a or miR-16 expression and lead to significant reduction in ovarian cancer cell proliferation and clonal growth. These findings suggest the development of therapeutic strategies by restoring miR-15a and miR-16 expression in ovarian cancer and in other cancers that involve upregulation of Bmi-1.

Original languageEnglish
Pages (from-to)9090-9095
Number of pages6
JournalCancer Research
Volume69
Issue number23
DOIs
Publication statusPublished - Dec 1 2009

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Ovarian Neoplasms
MicroRNAs
Cell Line
Neoplasms
3' Untranslated Regions
Proteins
Up-Regulation
Down-Regulation
Cell Proliferation
Growth
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bhattacharya, R., Nicoloso, M., Arvizo, R., Wang, E., Cortez, A., Rossi, S., ... Mukherjee, P. (2009). MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer. Cancer Research, 69(23), 9090-9095. https://doi.org/10.1158/0008-5472.CAN-09-2552

MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer. / Bhattacharya, Resham; Nicoloso, Milena; Arvizo, Rochelle; Wang, Enfeng; Cortez, Angelica; Rossi, Simona; Calin, George A.; Mukherjee, Priyabrata.

In: Cancer Research, Vol. 69, No. 23, 01.12.2009, p. 9090-9095.

Research output: Contribution to journalArticle

Bhattacharya, R, Nicoloso, M, Arvizo, R, Wang, E, Cortez, A, Rossi, S, Calin, GA & Mukherjee, P 2009, 'MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer', Cancer Research, vol. 69, no. 23, pp. 9090-9095. https://doi.org/10.1158/0008-5472.CAN-09-2552
Bhattacharya R, Nicoloso M, Arvizo R, Wang E, Cortez A, Rossi S et al. MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer. Cancer Research. 2009 Dec 1;69(23):9090-9095. https://doi.org/10.1158/0008-5472.CAN-09-2552
Bhattacharya, Resham ; Nicoloso, Milena ; Arvizo, Rochelle ; Wang, Enfeng ; Cortez, Angelica ; Rossi, Simona ; Calin, George A. ; Mukherjee, Priyabrata. / MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer. In: Cancer Research. 2009 ; Vol. 69, No. 23. pp. 9090-9095.
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