miR-181c associates with tumor relapse of high grade osteosarcoma

Mori Federica, Sacconi Andrea, Canu Valeria, Ganci Federica, Novello Mariangela, Anelli Vincenzo, Covello Renato, Ferraresi Virginia, Muti Paola, Biagini Roberto, Blandino Giovanni, Strano Sabrina

Research output: Contribution to journalArticlepeer-review

Abstract

High-grade osteosarcoma (OS) is characterized by low incidence, high aggressiveness and moderate 5-years survival rate after aggressive polychemotherapy and surgery. Here we used miRNA profiling as a tool to possibly predict and monitor OS's development and therapeutic outcome. First, we evaluated the altered expression of selected miRNAs from a case of Giant Cell Tumor (GCT) apparently evolved into an OS. We found that most of modulated miRs were associated with pathways of bone resorption and osteogenesis. miRNA expression also revealed that GCT and OS were distinct tumors. Second, we validated the observed miRNA profile in two independent casuistries of ten GCT (not evolved into malignant tumors) and sixteen OS patients. Interestingly, we found that miR-181c and other three miRNAs identified in the first step of the study were also consistently de-regulated in all OS patients. Ectopic expression of miR-181c reduced cell viability and enhanced chemotherapeutic-induced cell death of U2OS and SAOS2 cells. These findings indicate that: i) miRNAs aberrantly modulated in GCT could be predictive of its development into OS and ii) miRNAs expression could be useful to monitor the OS therapeutic outcome.

Original languageEnglish
Pages (from-to)13947-13961
Number of pages15
JournalOncotarget
Volume6
Issue number16
Publication statusPublished - 2015

Keywords

  • Giant cell tumor
  • miRNA profiling
  • Osteosarcoma
  • Relapse

ASJC Scopus subject areas

  • Oncology

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