MiR-184 expression is regulated by AMPK in pancreatic islets

A Martinez-Sanchez, MS Nguyen-Tu, I Cebola, A Yavari, P Marchetti, L Piemonti, E de Koning, AMJ Shapiro, P Johnson, K Sakamoto, DM Smith, I Leclerc, H Ashrafian, J Ferrer, GA Rutter

Research output: Contribution to journalArticlepeer-review


AMPK is a critical energy sensor and target for widely used antidiabetic drugs. In β-cells, elevated glucose concentrations lower AMPK activity, and the ablation of both catalytic subunits (βAMPKdKO mice) impairs insulin secretion in vivo and β-cell identity. MicroRNAs (miRNAs) are small RNAs that silence gene expression that are essential for pancreatic β-cell function and identity and altered in diabetes. Here, we have explored the miRNAs acting downstream of AMPK in mouse and human β-cells. We identified 14 down-regulated and 9 up-regulated miRNAs in βAMPKdKO vs. control islets. Gene ontology analysis of targeted transcripts revealed enrichment in pathways important for β-cell function and identity. The most down-regulated miRNA was miR-184 (miR-184-3p), an important regulator of β-cell function and compensatory expansion that is controlled by glucose and reduced in diabetes. We demonstrate that AMPK is a potent regulator and an important mediator of the negative effects of glucose on miR-184 expression. Additionally, we reveal sexual dimorphism in miR-184 expression in mouse and human islets. Collectively, these data demonstrate that glucose-mediated changes in AMPK activity are central for the regulation of miR-184 and other miRNAs in islets and provide a link between energy status and gene expression in β-cells.
Original languageEnglish
Pages (from-to)2587-2600
Number of pages14
JournalFASEB Journal
Issue number5
Publication statusPublished - 2018


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