MiR-187 targets the androgen-regulated gene ALDH1A3 in prostate cancer

Irene Casanova-Salas; Esther Masiá; Ana Armiñán; Ana Calatrava; Caterina Mancarella; José Rubio-Briones; Katia Scotlandi; Maria Jesús Vicent; José Antonio López-Guerrero

doi:10.1371/journal.pone.0125576

MiR-187 targets the androgen-regulated gene ALDH1A3 in prostate cancer

Irene Casanova-Salas, Esther Masiá, Ana Armiñán, Ana Calatrava, Caterina Mancarella, José Rubio-Briones, Katia Scotlandi, Maria Jesús Vicent, José Antonio López-Guerrero

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

miRNAs are predicted to control the activity of approximately 60% of all protein-coding genes participating in the regulation of several cellular processes and diseases, including cancer. Recently, we have demonstrated that miR-187 is significantly downregulated in prostate cancer (PCa) and here we propose a proteomic approach to identify its potential targets. For this purpose, PC-3 cells were transiently transfected with miR-187 precursor and miRNA mimic negative control. Proteins were analyzed by a two-dimensional difference gel electrophoresis (2D-DIGE) and defined as differentially regulated if the observed fold change was ±1.06. Then, MALDI-TOF MS analysis was performed after protein digestion and low abundance proteins were identified by LC-MS/MS. Peptides were identified by searching against the Expasy SWISS PROT database, and target validation was performed both in vitro by western blot and qRT-PCR and in clinical samples by qRT-PCR, immunohistochemistry and ELISA. DIGE analysis showed 9 differentially expressed spots (p

Original language	English
Article number	e0125576
Journal	PLoS One
Volume	10
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0125576
Publication status	Published - May 13 2015

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)

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MiR-187 targets the androgen-regulated gene ALDH1A3 in prostate cancer. / Casanova-Salas, Irene; Masiá, Esther; Armiñán, Ana; Calatrava, Ana; Mancarella, Caterina; Rubio-Briones, José; Scotlandi, Katia; Vicent, Maria Jesús; López-Guerrero, José Antonio.

In: PLoS One, Vol. 10, No. 5, e0125576, 13.05.2015.

Research output: Contribution to journal › Article › peer-review

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AU - Calatrava, Ana

AU - Mancarella, Caterina

AU - Rubio-Briones, José

AU - Scotlandi, Katia

AU - Vicent, Maria Jesús

AU - López-Guerrero, José Antonio

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