MiR-187 targets the androgen-regulated gene ALDH1A3 in prostate cancer

Irene Casanova-Salas, Esther Masiá, Ana Armiñán, Ana Calatrava, Caterina Mancarella, José Rubio-Briones, Katia Scotlandi, Maria Jesús Vicent, José Antonio López-Guerrero

Research output: Contribution to journalArticlepeer-review

Abstract

miRNAs are predicted to control the activity of approximately 60% of all protein-coding genes participating in the regulation of several cellular processes and diseases, including cancer. Recently, we have demonstrated that miR-187 is significantly downregulated in prostate cancer (PCa) and here we propose a proteomic approach to identify its potential targets. For this purpose, PC-3 cells were transiently transfected with miR-187 precursor and miRNA mimic negative control. Proteins were analyzed by a two-dimensional difference gel electrophoresis (2D-DIGE) and defined as differentially regulated if the observed fold change was ±1.06. Then, MALDI-TOF MS analysis was performed after protein digestion and low abundance proteins were identified by LC-MS/MS. Peptides were identified by searching against the Expasy SWISS PROT database, and target validation was performed both in vitro by western blot and qRT-PCR and in clinical samples by qRT-PCR, immunohistochemistry and ELISA. DIGE analysis showed 9 differentially expressed spots (p

Original languageEnglish
Article numbere0125576
JournalPLoS One
Volume10
Issue number5
DOIs
Publication statusPublished - May 13 2015

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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