MiR-203 controls proliferation, migration and invasive potential of prostate cancer cell lines

Giuditta Viticchiè, Anna Maria Lena, Alessia Latina, Amanda Formosa, Lea H. Gregersen, Anders H. Lund, Sergio Bernardini, Alessandro Mauriello, Roberto Miano, Luigi G. Spagnoli, Richard A. Knight, Eleonora Candi, Gerry Melino

Research output: Contribution to journalArticlepeer-review

Abstract

Prostate cancers show a slow progression from a local lesion (primary tumor) to a metastatic and hormone-resistant phenotype. After an initial step of hyperplasia, in a high percentage of cases a neoplastic transformation event occurs that, less frequently, is followed by epithelial to mesenchymal transition and invasion of healthy tissues (usually bones). MicroRNA-203 (miR-203) is a tumor suppressor microRNA often silenced in different malignancies. Here, we show that miR-203 is downregulated in clinical primary prostatic tumors compared to normal prostate tissue and in metastatic prostate cancer cell lines compared to normal epithelial prostatic cells. Overexpression of miR-203 in brain or bone metastatic prostate cell lines (DU145 and PC3) is sufficient to induce a mesenchymal to epithelial transition with inhibition of cell proliferation, migration and invasiveness. We have identified CKAP2, LASP1, BIRC5, WASF1, ASAP1 and RUNX2 as new miR-203 direct target mRNAs involved in these events. Therefore, miR-203 could be a potentially new prognostic marker and therapeutic target in metastatic prostate cancer.

Original languageEnglish
Pages (from-to)1121-1131
Number of pages11
JournalCell Cycle
Volume10
Issue number7
DOIs
Publication statusPublished - Apr 1 2011

Keywords

  • EMT
  • Invasion
  • Metastasis
  • Migration
  • miR-203
  • Proliferation
  • Prostate cancer

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Fingerprint Dive into the research topics of 'MiR-203 controls proliferation, migration and invasive potential of prostate cancer cell lines'. Together they form a unique fingerprint.

Cite this