Mir-223 regulates the number and function of myeloid-derived suppressor cells in multiple sclerosis and experimental autoimmune encephalomyelitis

Claudia Cantoni, Francesca Cignarella, Laura Ghezzi, Bob Mikesell, Bryan Bollman, Melissa M. Berrien-Elliott, Aaron R. Ireland, Todd A. Fehniger, Gregory F. Wu, Laura Piccio

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Myeloid-derived cells play important modulatory and effector roles in multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells, composed of monocytic (MO) and polymorphonuclear (PMN) fractions, which can suppress T cell activities in EAE. Their role in MS remains poorly characterized. We found decreased numbers of circulating MDSCs, driven by lower frequencies of the MO-MDSCs, and higher MDSC expression of microRNA miR-223 in MS versus healthy subjects. To gain mechanistic insights, we interrogated the EAE model. MiR-223 knock out (miR-223−/−) mice developed less severe EAE with increased MDSC numbers in the spleen and spinal cord compared to littermate controls. MiR-223−/− MO-MDSCs suppressed T cell proliferation and cytokine production in vitro and EAE in vivo more than wild-type MO-MDSCs. They also displayed an increased expression of critical mediators of MDSC suppressive function, Arginase-1(Arg1), and the signal transducer and activator of transcription 3 (Stat3), which herein, we demonstrate being an miR-223 target gene. Consistently, MDSCs from MS patients displayed decreased STAT3 and ARG1 expression compared with healthy controls, suggesting that circulating MDSCs in MS are not only reduced in numbers but also less suppressive. These results support a critical role for miR-223 in modulating MDSC biology in EAE and in MS and suggest potential novel therapeutic applications.

Original languageEnglish
Pages (from-to)61-77
Number of pages17
JournalActa Neuropathologica
Volume133
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Autoimmune Experimental Encephalomyelitis
Multiple Sclerosis
Myeloid Cells
Myeloid-Derived Suppressor Cells
T-Lymphocytes
Arginase
STAT3 Transcription Factor
MicroRNAs
Knockout Mice
Cell Biology
Spinal Cord
Healthy Volunteers
Spleen
Animal Models
Cell Count
Cell Proliferation
Cytokines

Keywords

  • MicroRNA
  • MiR-223
  • Multiple sclerosis
  • Myeloid-derived suppressor cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Mir-223 regulates the number and function of myeloid-derived suppressor cells in multiple sclerosis and experimental autoimmune encephalomyelitis. / Cantoni, Claudia; Cignarella, Francesca; Ghezzi, Laura; Mikesell, Bob; Bollman, Bryan; Berrien-Elliott, Melissa M.; Ireland, Aaron R.; Fehniger, Todd A.; Wu, Gregory F.; Piccio, Laura.

In: Acta Neuropathologica, Vol. 133, No. 1, 2017, p. 61-77.

Research output: Contribution to journalArticle

Cantoni, C, Cignarella, F, Ghezzi, L, Mikesell, B, Bollman, B, Berrien-Elliott, MM, Ireland, AR, Fehniger, TA, Wu, GF & Piccio, L 2017, 'Mir-223 regulates the number and function of myeloid-derived suppressor cells in multiple sclerosis and experimental autoimmune encephalomyelitis', Acta Neuropathologica, vol. 133, no. 1, pp. 61-77. https://doi.org/10.1007/s00401-016-1621-6
Cantoni, Claudia ; Cignarella, Francesca ; Ghezzi, Laura ; Mikesell, Bob ; Bollman, Bryan ; Berrien-Elliott, Melissa M. ; Ireland, Aaron R. ; Fehniger, Todd A. ; Wu, Gregory F. ; Piccio, Laura. / Mir-223 regulates the number and function of myeloid-derived suppressor cells in multiple sclerosis and experimental autoimmune encephalomyelitis. In: Acta Neuropathologica. 2017 ; Vol. 133, No. 1. pp. 61-77.
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