miR-2861 is involved in osteogenic commitment of human periodontal ligament stem cells grown onto 3D scaffold

Francesca Diomede, Ilaria Merciaro, A S Martinotti, M F X B Cavalcanti, Sergio Caputi, E Mazzon, O. Trubiani

Research output: Contribution to journalArticle

Abstract

miR-2861 endorsing osteoblast differentiation through the overexpression of Runt-related transcription factor 2 (RUNX2) protein has been recently described. In this study we evaluated: the performance of living construct, composed by human Periodontal Ligament Stem Cells (hPDLSCs) and 3D scaffold (EXg), and the behaviour of miR-2861/RUNX2 expression pathway on the osteogenic commitment. Human PDLSCs were seeded with and without EXg scaffold and cultured under basal and osteogenic conditions. Morphological features, adhesiveness and differentiation abilities were analysed using scanning electron and confocal laser scanning microscopy. Time-course of RUNX2, ALP, OPN and miR-2861 were evaluated through RT-PCR analysis. Our results highlighted that the osteogenic differentiation was mostly obvious in the hPDLSCs, grown onto 3D scaffold in presence of osteoinductive medium. Moreover, the overexpression of miR-2861 and RUNX2 in hPDLSCs cultured in presence of EXg under osteogenic and standard conditions was demonstrated. In synthesis, the increased expression of miR-2861/RUNX2 provides new insights regarding miRNA signaling network in the presence of scaffold providing an additional method to evaluate the performance of biomaterial in bone regeneration.

Original languageEnglish
Pages (from-to)1009-1018
Number of pages10
JournalJournal of Biological Regulators and Homeostatic Agents
Volume30
Issue number4
Publication statusE-pub ahead of print - Oct 2016

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Periodontal Ligament
Stem Cells
Adhesiveness
Bone Regeneration
Aptitude
Biocompatible Materials
Osteoblasts
MicroRNAs
Confocal Microscopy
Transcription Factors
Electrons
Polymerase Chain Reaction
Proteins

Keywords

  • Journal Article

Cite this

Diomede, F., Merciaro, I., Martinotti, A. S., Cavalcanti, M. F. X. B., Caputi, S., Mazzon, E., & Trubiani, O. (2016). miR-2861 is involved in osteogenic commitment of human periodontal ligament stem cells grown onto 3D scaffold. Journal of Biological Regulators and Homeostatic Agents, 30(4), 1009-1018.

miR-2861 is involved in osteogenic commitment of human periodontal ligament stem cells grown onto 3D scaffold. / Diomede, Francesca; Merciaro, Ilaria; Martinotti, A S; Cavalcanti, M F X B; Caputi, Sergio; Mazzon, E; Trubiani, O.

In: Journal of Biological Regulators and Homeostatic Agents, Vol. 30, No. 4, 10.2016, p. 1009-1018.

Research output: Contribution to journalArticle

Diomede, F, Merciaro, I, Martinotti, AS, Cavalcanti, MFXB, Caputi, S, Mazzon, E & Trubiani, O 2016, 'miR-2861 is involved in osteogenic commitment of human periodontal ligament stem cells grown onto 3D scaffold', Journal of Biological Regulators and Homeostatic Agents, vol. 30, no. 4, pp. 1009-1018.
Diomede, Francesca ; Merciaro, Ilaria ; Martinotti, A S ; Cavalcanti, M F X B ; Caputi, Sergio ; Mazzon, E ; Trubiani, O. / miR-2861 is involved in osteogenic commitment of human periodontal ligament stem cells grown onto 3D scaffold. In: Journal of Biological Regulators and Homeostatic Agents. 2016 ; Vol. 30, No. 4. pp. 1009-1018.
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AU - Caputi, Sergio

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AB - miR-2861 endorsing osteoblast differentiation through the overexpression of Runt-related transcription factor 2 (RUNX2) protein has been recently described. In this study we evaluated: the performance of living construct, composed by human Periodontal Ligament Stem Cells (hPDLSCs) and 3D scaffold (EXg), and the behaviour of miR-2861/RUNX2 expression pathway on the osteogenic commitment. Human PDLSCs were seeded with and without EXg scaffold and cultured under basal and osteogenic conditions. Morphological features, adhesiveness and differentiation abilities were analysed using scanning electron and confocal laser scanning microscopy. Time-course of RUNX2, ALP, OPN and miR-2861 were evaluated through RT-PCR analysis. Our results highlighted that the osteogenic differentiation was mostly obvious in the hPDLSCs, grown onto 3D scaffold in presence of osteoinductive medium. Moreover, the overexpression of miR-2861 and RUNX2 in hPDLSCs cultured in presence of EXg under osteogenic and standard conditions was demonstrated. In synthesis, the increased expression of miR-2861/RUNX2 provides new insights regarding miRNA signaling network in the presence of scaffold providing an additional method to evaluate the performance of biomaterial in bone regeneration.

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