MiR-375 and YAP1 expression profiling in medullary thyroid carcinoma and their correlation with clinical–pathological features and outcome

Francesca Galuppini, Loris Bertazza, Susi Barollo, Elisabetta Cavedon, Massimo Rugge, Vincenza Guzzardo, Diana Sacchi, Sara Watutantrige-Fernando, Federica Vianello, Caterina Mian, Gianmaria Pennelli

Research output: Contribution to journalArticlepeer-review

Abstract

Medullary thyroid cancer (MTC) is a tumor marked by an indolent growth for which few prognostic factors and therapeutic strategies are actually available. Different studies have recently appraised well-differentiated thyroid cancers are characterized by a dysregulation in different microRNA sets; however, only few of them investigated the role of miRNA expression in MTCs. In this study, we have assessed the miR-375 expression in a series of 130 MTCs (104 are sporadic and 26 familial) with a median follow-up of 39 months (range 1–138) and then we have correlated our results with the clinical–pathological features and the patients’ outcome. Moreover, we have appraised YAP1 (Yes-associated protein 1) immunohistochemical expression in the same MTC series and in 5 C-cells hyperplasia (CCH) samples as well. We observed a significant upregulation of miR-375 in all MTCs, when compared to the normal thyroid tissues. Besides, miR-375 expression was found to be closely linked to neoplastic size, a chance of thyroid capsule infiltration, the risk of lymph node metastasis, and the staging of the tumor. At the end of follow-up, only 10% (13/130) showed a tumor progression and a higher miR-375 expression was found to be closely linked to a worst patient’ outcome. On the contrary, YAP1 immunohistochemical expression was sharply downregulated in tumors, whereas it was weakly expressed in CCHs. Our results suggest miR-375 plays a central role in MTC progression and, therefore, we seek following the idea that miR-375 pathway may be an effective target in novel MTC therapeutic strategies.

Original languageEnglish
Pages (from-to)651-658
Number of pages8
JournalVirchows Archiv
Volume471
Issue number5
DOIs
Publication statusPublished - Nov 1 2017

Keywords

  • Medullary thyroid cancer
  • MicroRNA
  • miR-375
  • YAP1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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