Abstract
The survival rates in colon cancer patients are inversely proportional to the number of lymph node metastases. The hypoxia-induced Epithelial to Mesenchymal Transition (EMT), driven by HIF1α, is known to be involved in cancer progression and metastasis. Recently, we have reported that miR-675-5p promotes glioma growth by stabilizing HIF1α here, by use of the syngeneic cell lines we investigated the role of the miR-675-5p in colon cancer metastasis. Our results show that miR-675-5p, over expressed in metastatic colon cancer cells, participates to tumour progression by regulating HIF1α induced EMT. MiR-675- 5p increases Snail transcription by a dual strategy: i) stabilizing the activity of the transcription factor HIF1α and ii) and inhibiting Snail's repressor DDB2 (Damage specific DNA Binding protein 2). Moreover, transcriptional analyses on specimens from colon cancer patients confirmed, in vivo, the correlation between miR-675-5p over-expression and metastasis, thus identifying miR-675-5p as a new marker for colon cancer progression and therefore a putative target for therapeutic strategies.
| Original language | English |
|---|---|
| Pages (from-to) | 24292-24302 |
| Number of pages | 11 |
| Journal | Oncotarget |
| Volume | 8 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Jan 1 2017 |
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Keywords
- CRC
- EMT
- Hypoxia
- Metastasis
- MiRNA675
ASJC Scopus subject areas
- Oncology
Cite this
MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells. / Costa, Viviana; Lo Dico, Alessia; Rizzo, Aroldo; Rajata, Francesca; Tripodi, Marco; Alessandro, Riccardo; Conigliaro, Alice.
In: Oncotarget, Vol. 8, No. 15, 01.01.2017, p. 24292-24302.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MiR-675-5p supports hypoxia induced epithelial to mesenchymal transition in colon cancer cells
AU - Costa, Viviana
AU - Lo Dico, Alessia
AU - Rizzo, Aroldo
AU - Rajata, Francesca
AU - Tripodi, Marco
AU - Alessandro, Riccardo
AU - Conigliaro, Alice
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The survival rates in colon cancer patients are inversely proportional to the number of lymph node metastases. The hypoxia-induced Epithelial to Mesenchymal Transition (EMT), driven by HIF1α, is known to be involved in cancer progression and metastasis. Recently, we have reported that miR-675-5p promotes glioma growth by stabilizing HIF1α here, by use of the syngeneic cell lines we investigated the role of the miR-675-5p in colon cancer metastasis. Our results show that miR-675-5p, over expressed in metastatic colon cancer cells, participates to tumour progression by regulating HIF1α induced EMT. MiR-675- 5p increases Snail transcription by a dual strategy: i) stabilizing the activity of the transcription factor HIF1α and ii) and inhibiting Snail's repressor DDB2 (Damage specific DNA Binding protein 2). Moreover, transcriptional analyses on specimens from colon cancer patients confirmed, in vivo, the correlation between miR-675-5p over-expression and metastasis, thus identifying miR-675-5p as a new marker for colon cancer progression and therefore a putative target for therapeutic strategies.
AB - The survival rates in colon cancer patients are inversely proportional to the number of lymph node metastases. The hypoxia-induced Epithelial to Mesenchymal Transition (EMT), driven by HIF1α, is known to be involved in cancer progression and metastasis. Recently, we have reported that miR-675-5p promotes glioma growth by stabilizing HIF1α here, by use of the syngeneic cell lines we investigated the role of the miR-675-5p in colon cancer metastasis. Our results show that miR-675-5p, over expressed in metastatic colon cancer cells, participates to tumour progression by regulating HIF1α induced EMT. MiR-675- 5p increases Snail transcription by a dual strategy: i) stabilizing the activity of the transcription factor HIF1α and ii) and inhibiting Snail's repressor DDB2 (Damage specific DNA Binding protein 2). Moreover, transcriptional analyses on specimens from colon cancer patients confirmed, in vivo, the correlation between miR-675-5p over-expression and metastasis, thus identifying miR-675-5p as a new marker for colon cancer progression and therefore a putative target for therapeutic strategies.
KW - CRC
KW - EMT
KW - Hypoxia
KW - Metastasis
KW - MiRNA675
UR - http://www.scopus.com/inward/record.url?scp=85017542064&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017542064&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.14464
DO - 10.18632/oncotarget.14464
M3 - Article
AN - SCOPUS:85017542064
VL - 8
SP - 24292
EP - 24302
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 15
ER -