TY - JOUR
T1 - MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease
AU - Serpente, Maria
AU - Fenoglio, Chiara
AU - D'Anca, Marianna
AU - Arcaro, Marina
AU - Sorrentino, Federica
AU - Visconte, Caterina
AU - Arighi, Andrea
AU - Fumagalli, Giorgio G.
AU - Porretti, Laura
AU - Cattaneo, Alessandra
AU - Ciani, Miriam
AU - Zanardini, Roberta
AU - Benussi, Luisa
AU - Ghidoni, Roberta
AU - Scarpini, Elio
AU - Galimberti, Daniela
PY - 2020/6/10
Y1 - 2020/6/10
N2 - Small extracellular vesicles (EVs) are able to pass from the central nervous system (CNS) into peripheral blood and contain molecule markers of their parental origin. The aim of our study was to isolate and characterize total and neural-derived small EVs (NDEVs) and their micro RNA (miRNA) cargo in Alzheimer's disease (AD) patients. Small NDEVs were isolated from plasma in a population consisting of 40 AD patients and 40 healthy subjects (CTRLs) using high throughput Advanced TaqMan miRNA OpenArrays®, which enables the simultaneous determination of 754 miRNAs. MiR-23a-3p, miR-223-3p, miR-100-3p and miR-190-5p showed a significant dysregulation in small NDEVs from AD patients as compared with controls (1.16 ± 0.49 versus 7.54 ± 2.5, p = 0.026; 9.32 ± 2.27 versus 0.66 ± 0.18, p <0.0001; 0.069 ± 0.01 versus 0.5 ± 0.1, p < 0.0001 and 2.9 ± 1.2 versus 1.93 ± 0.9, p < 0.05, respectively). A further validation analysis confirmed that miR-23a-3p, miR-223-3p and miR-190a-5p levels in small NDEVs from AD patients were significantly upregulated as compared with controls (p = 0.008; p = 0.016; p = 0.003, respectively) whereas miR-100-3p levels were significantly downregulated (p = 0.008). This is the first study that carries out the comparison between total plasma small EV population and NDEVs, demonstrating the presence of a specific AD NDEV miRNA signature.
AB - Small extracellular vesicles (EVs) are able to pass from the central nervous system (CNS) into peripheral blood and contain molecule markers of their parental origin. The aim of our study was to isolate and characterize total and neural-derived small EVs (NDEVs) and their micro RNA (miRNA) cargo in Alzheimer's disease (AD) patients. Small NDEVs were isolated from plasma in a population consisting of 40 AD patients and 40 healthy subjects (CTRLs) using high throughput Advanced TaqMan miRNA OpenArrays®, which enables the simultaneous determination of 754 miRNAs. MiR-23a-3p, miR-223-3p, miR-100-3p and miR-190-5p showed a significant dysregulation in small NDEVs from AD patients as compared with controls (1.16 ± 0.49 versus 7.54 ± 2.5, p = 0.026; 9.32 ± 2.27 versus 0.66 ± 0.18, p <0.0001; 0.069 ± 0.01 versus 0.5 ± 0.1, p < 0.0001 and 2.9 ± 1.2 versus 1.93 ± 0.9, p < 0.05, respectively). A further validation analysis confirmed that miR-23a-3p, miR-223-3p and miR-190a-5p levels in small NDEVs from AD patients were significantly upregulated as compared with controls (p = 0.008; p = 0.016; p = 0.003, respectively) whereas miR-100-3p levels were significantly downregulated (p = 0.008). This is the first study that carries out the comparison between total plasma small EV population and NDEVs, demonstrating the presence of a specific AD NDEV miRNA signature.
KW - Alzheimer’s disease
KW - extracellular vesicles (EVs)
KW - microRNA
KW - neural-derived extracellular vesicles (NDEVs)
UR - http://www.scopus.com/inward/record.url?scp=85086607044&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85086607044&partnerID=8YFLogxK
U2 - 10.3390/cells9061443
DO - 10.3390/cells9061443
M3 - Article
C2 - 32531989
AN - SCOPUS:85086607044
VL - 9
JO - Cells
JF - Cells
SN - 2073-4409
IS - 6
M1 - 1443
ER -