MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease

M. Serpente, C. Fenoglio, M. D'Anca, M. Arcaro, F. Sorrentino, C. Visconte, A. Arighi, G.G. Fumagalli, L. Porretti, A. Cattaneo, M. Ciani, R. Zanardini, L. Benussi, R. Ghidoni, E. Scarpini, D. Galimberti

Research output: Contribution to journalArticlepeer-review

Abstract

Small extracellular vesicles (EVs) are able to pass from the central nervous system (CNS) into peripheral blood and contain molecule markers of their parental origin. The aim of our study was to isolate and characterize total and neural-derived small EVs (NDEVs) and their micro RNA (miRNA) cargo in Alzheimer's disease (AD) patients. Small NDEVs were isolated from plasma in a population consisting of 40 AD patients and 40 healthy subjects (CTRLs) using high throughput Advanced TaqMan miRNA OpenArrays®, which enables the simultaneous determination of 754 miRNAs. MiR-23a-3p, miR-223-3p, miR-100-3p and miR-190-5p showed a significant dysregulation in small NDEVs from AD patients as compared with controls (1.16 ± 0.49 versus 7.54 ± 2.5, p = 0.026; 9.32 ± 2.27 versus 0.66 ± 0.18, p
Original languageEnglish
Article numberE1443
JournalCells
Volume9
Issue number6
DOIs
Publication statusPublished - 2020

Keywords

  • Alzheimer’s disease
  • extracellular vesicles (EVs)
  • microRNA
  • neural-derived extracellular vesicles (NDEVs)

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