MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease

M. Serpente; C. Fenoglio; M. D'Anca; M. Arcaro; F. Sorrentino; C. Visconte; A. Arighi; G.G. Fumagalli; L. Porretti; A. Cattaneo; M. Ciani; R. Zanardini; L. Benussi; R. Ghidoni; E. Scarpini; D. Galimberti

doi:10.3390/cells9061443

MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease

M. Serpente, C. Fenoglio, M. D'Anca, M. Arcaro, F. Sorrentino, C. Visconte, A. Arighi, G.G. Fumagalli, L. Porretti, A. Cattaneo, M. Ciani, R. Zanardini, L. Benussi, R. Ghidoni, E. Scarpini, D. Galimberti

Centro San Giovanni di Dio - Fatebenefratelli

Research output: Contribution to journal › Article › peer-review

Abstract

Small extracellular vesicles (EVs) are able to pass from the central nervous system (CNS) into peripheral blood and contain molecule markers of their parental origin. The aim of our study was to isolate and characterize total and neural-derived small EVs (NDEVs) and their micro RNA (miRNA) cargo in Alzheimer's disease (AD) patients. Small NDEVs were isolated from plasma in a population consisting of 40 AD patients and 40 healthy subjects (CTRLs) using high throughput Advanced TaqMan miRNA OpenArrays®, which enables the simultaneous determination of 754 miRNAs. MiR-23a-3p, miR-223-3p, miR-100-3p and miR-190-5p showed a significant dysregulation in small NDEVs from AD patients as compared with controls (1.16 ± 0.49 versus 7.54 ± 2.5, p = 0.026; 9.32 ± 2.27 versus 0.66 ± 0.18, p

Original language	English
Article number	E1443
Journal	Cells
Volume	9
Issue number	6
DOIs	https://doi.org/10.3390/cells9061443
Publication status	Published - 2020

Keywords

Alzheimer’s disease
extracellular vesicles (EVs)
microRNA
neural-derived extracellular vesicles (NDEVs)

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10.3390/cells9061443

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086607044&doi=10.3390%2fcells9061443&partnerID=40&md5=b5bf30218ff52415804597163224a0e7

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Serpente, M., Fenoglio, C., D'Anca, M., Arcaro, M., Sorrentino, F., Visconte, C., Arighi, A., Fumagalli, G. G., Porretti, L., Cattaneo, A., Ciani, M., Zanardini, R., Benussi, L., Ghidoni, R., Scarpini, E., & Galimberti, D. (2020). MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease. Cells, 9(6), [E1443]. https://doi.org/10.3390/cells9061443

MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease. / Serpente, M.; Fenoglio, C.; D'Anca, M.; Arcaro, M.; Sorrentino, F.; Visconte, C.; Arighi, A.; Fumagalli, G.G.; Porretti, L.; Cattaneo, A.; Ciani, M.; Zanardini, R.; Benussi, L.; Ghidoni, R.; Scarpini, E.; Galimberti, D.

In: Cells, Vol. 9, No. 6, E1443, 2020.

Research output: Contribution to journal › Article › peer-review

Serpente, M, Fenoglio, C, D'Anca, M, Arcaro, M, Sorrentino, F, Visconte, C, Arighi, A, Fumagalli, GG, Porretti, L, Cattaneo, A, Ciani, M , Zanardini, R , Benussi, L , Ghidoni, R, Scarpini, E & Galimberti, D 2020, 'MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease', Cells, vol. 9, no. 6, E1443. https://doi.org/10.3390/cells9061443

Serpente, M. ; Fenoglio, C. ; D'Anca, M. ; Arcaro, M. ; Sorrentino, F. ; Visconte, C. ; Arighi, A. ; Fumagalli, G.G. ; Porretti, L. ; Cattaneo, A. ; Ciani, M. ; Zanardini, R. ; Benussi, L. ; Ghidoni, R. ; Scarpini, E. ; Galimberti, D. / MiRNA Profiling in Plasma Neural-Derived Small Extracellular Vesicles from Patients with Alzheimer's Disease. In: Cells. 2020 ; Vol. 9, No. 6.

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abstract = "Small extracellular vesicles (EVs) are able to pass from the central nervous system (CNS) into peripheral blood and contain molecule markers of their parental origin. The aim of our study was to isolate and characterize total and neural-derived small EVs (NDEVs) and their micro RNA (miRNA) cargo in Alzheimer's disease (AD) patients. Small NDEVs were isolated from plasma in a population consisting of 40 AD patients and 40 healthy subjects (CTRLs) using high throughput Advanced TaqMan miRNA OpenArrays{\textregistered}, which enables the simultaneous determination of 754 miRNAs. MiR-23a-3p, miR-223-3p, miR-100-3p and miR-190-5p showed a significant dysregulation in small NDEVs from AD patients as compared with controls (1.16 ± 0.49 versus 7.54 ± 2.5, p = 0.026; 9.32 ± 2.27 versus 0.66 ± 0.18, p ",

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AU - Serpente, M.

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AU - Arcaro, M.

AU - Sorrentino, F.

AU - Visconte, C.

AU - Arighi, A.

AU - Fumagalli, G.G.

AU - Porretti, L.

AU - Cattaneo, A.

AU - Ciani, M.

AU - Zanardini, R.

AU - Benussi, L.

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AU - Galimberti, D.

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AB - Small extracellular vesicles (EVs) are able to pass from the central nervous system (CNS) into peripheral blood and contain molecule markers of their parental origin. The aim of our study was to isolate and characterize total and neural-derived small EVs (NDEVs) and their micro RNA (miRNA) cargo in Alzheimer's disease (AD) patients. Small NDEVs were isolated from plasma in a population consisting of 40 AD patients and 40 healthy subjects (CTRLs) using high throughput Advanced TaqMan miRNA OpenArrays®, which enables the simultaneous determination of 754 miRNAs. MiR-23a-3p, miR-223-3p, miR-100-3p and miR-190-5p showed a significant dysregulation in small NDEVs from AD patients as compared with controls (1.16 ± 0.49 versus 7.54 ± 2.5, p = 0.026; 9.32 ± 2.27 versus 0.66 ± 0.18, p

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