MiRNAs as potential prognostic biomarkers for metastasis in thin and thick primary cutaneous melanomas

Virginia Valentini, Veronica Zelli, Emanuela Gaggiano, Valentina Silvestri, Piera Rizzolo, Agostino Bucalo, Stefano Calvieri, Sara Grassi, Pasquale Frascione, Pietro Donati, Giuseppe Soda, Laura Ottini, Antonio Giovanni Richetta

Research output: Contribution to journalArticle

Abstract

Background/Aim: The identification of novel prognostic biomarkers for melanoma metastasis is essential to improve patient outcomes. To this aim, we characterized miRNA expression profiles in relation to metastasis in melanoma and correlated miRNAs expression with clinical-pathological factors. Materials and Methods: MiR-145-5p, miR-150-5p, miR-182-5p, miR-203-3p, miR-205-5p and miR-211-5p expression levels were analyzed in primary cutaneous melanomas, including thin and thick melanomas, and in melanoma metastases by quantitative Real-Time PCR. Results: A significantly lower miR-205-5p expression was found in metastases compared to primary melanomas. Furthermore, a progressive down-regulation of miR-205-5p expression was observed from loco-regional to distant metastasis. Significantly lower miR-145-5p and miR-203-3p expression levels were found in cases with Breslow thickness >1 mm, high Clark level, ulceration and mitotic rate ≥1/mm2. Conclusion: Our findings point to miR-205-5p as potential biomarker of distant metastases and to miR-145-5p and miR-203-3p as markers of aggressiveness in melanoma.

Original languageEnglish
Pages (from-to)4085-4093
Number of pages9
JournalAnticancer Research
Volume39
Issue number8
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • Cutaneous melanoma
  • Metastasis
  • Metastatic melanoma
  • MicroRNAs
  • Molecular biomarkers
  • Thick melanoma
  • Thin melanoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Valentini, V., Zelli, V., Gaggiano, E., Silvestri, V., Rizzolo, P., Bucalo, A., Calvieri, S., Grassi, S., Frascione, P., Donati, P., Soda, G., Ottini, L., & Richetta, A. G. (2019). MiRNAs as potential prognostic biomarkers for metastasis in thin and thick primary cutaneous melanomas. Anticancer Research, 39(8), 4085-4093. https://doi.org/10.21873/anticanres.13566