Abstract
Graft-versus-Host disease (GVHD) has been widely linked to immunogenetic causes such as disparity between the recipient and its HLA geno-identical donor for some Non-HLA antigens called minor histocompatibility antigens (MiHAgs). HA-2 is one of potential human MiHAgs but its effect on the GVHD occurrence remains not clear. In order to examine such association in the Tunisian cohort of HSCs recipients, we performed a retrospective study on patients who received an HLA-identical HSCT between 2000 and 2009. The study was performed on 60 HLA-A2-positive patients who had received a haematopoietic stem cell transplant from an HLA-identical sibling. All patients received cyclosporine A andor methotrexate for GVHD prophylaxis. HA-2 genotyping assay was performed with SSP-PCR method and HLA-A*0201 positive samples were identified mainly with Luminex HLA-Typing method. Luminex HLA-Typing assay showed that only 53 cases were positives for the HLA-A*0201 allele. Among these cases, only 3 pairs were mismatched for the MiHAg HA-2. Acute GVHD occurred in 01 HA-2-mismatched pair while chronic GVHD was detected in 02 disparate couples. Univariate and multivariate analyses showed that MiHAg HA-2 disparity does not have any significant effect on the occurrence of either acute or chronic GVHD. This last one appeared to be correlated only with the age of patient (adulthood) (p: 0.011, OR: 22.092). Our findings support the previously reported data denying the influence of the HA-2 disparity on the GVHD occurrence after HSCT.
| Original language | English |
|---|---|
| Pages (from-to) | 611-620 |
| Number of pages | 10 |
| Journal | Immunological Investigations |
| Volume | 39 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2010 |
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Keywords
- Graft-vs-Host disease
- HA-2
- Haematopoietic Stem Cells Transplantation
- HLA-A*0201
- Minor histocompatibility antigens
- Tunisian population
ASJC Scopus subject areas
- Immunology
Cite this
Mismatch for the minor histocompatibility antigen HA-2 and GVHD occurrence in HLA-A*0201-positive tunisian recipients of HSCs. / Sellami, Mohamed Hichem; Torjemane, Lamia; De Arias, Alejandro Espadas; Kaabi, Houda; Ladeb, Saloua; Ben Othman, Tarek; Poli, Francesca; Hmida, Slama.
In: Immunological Investigations, Vol. 39, No. 6, 2010, p. 611-620.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mismatch for the minor histocompatibility antigen HA-2 and GVHD occurrence in HLA-A*0201-positive tunisian recipients of HSCs
AU - Sellami, Mohamed Hichem
AU - Torjemane, Lamia
AU - De Arias, Alejandro Espadas
AU - Kaabi, Houda
AU - Ladeb, Saloua
AU - Ben Othman, Tarek
AU - Poli, Francesca
AU - Hmida, Slama
PY - 2010
Y1 - 2010
N2 - Graft-versus-Host disease (GVHD) has been widely linked to immunogenetic causes such as disparity between the recipient and its HLA geno-identical donor for some Non-HLA antigens called minor histocompatibility antigens (MiHAgs). HA-2 is one of potential human MiHAgs but its effect on the GVHD occurrence remains not clear. In order to examine such association in the Tunisian cohort of HSCs recipients, we performed a retrospective study on patients who received an HLA-identical HSCT between 2000 and 2009. The study was performed on 60 HLA-A2-positive patients who had received a haematopoietic stem cell transplant from an HLA-identical sibling. All patients received cyclosporine A andor methotrexate for GVHD prophylaxis. HA-2 genotyping assay was performed with SSP-PCR method and HLA-A*0201 positive samples were identified mainly with Luminex HLA-Typing method. Luminex HLA-Typing assay showed that only 53 cases were positives for the HLA-A*0201 allele. Among these cases, only 3 pairs were mismatched for the MiHAg HA-2. Acute GVHD occurred in 01 HA-2-mismatched pair while chronic GVHD was detected in 02 disparate couples. Univariate and multivariate analyses showed that MiHAg HA-2 disparity does not have any significant effect on the occurrence of either acute or chronic GVHD. This last one appeared to be correlated only with the age of patient (adulthood) (p: 0.011, OR: 22.092). Our findings support the previously reported data denying the influence of the HA-2 disparity on the GVHD occurrence after HSCT.
AB - Graft-versus-Host disease (GVHD) has been widely linked to immunogenetic causes such as disparity between the recipient and its HLA geno-identical donor for some Non-HLA antigens called minor histocompatibility antigens (MiHAgs). HA-2 is one of potential human MiHAgs but its effect on the GVHD occurrence remains not clear. In order to examine such association in the Tunisian cohort of HSCs recipients, we performed a retrospective study on patients who received an HLA-identical HSCT between 2000 and 2009. The study was performed on 60 HLA-A2-positive patients who had received a haematopoietic stem cell transplant from an HLA-identical sibling. All patients received cyclosporine A andor methotrexate for GVHD prophylaxis. HA-2 genotyping assay was performed with SSP-PCR method and HLA-A*0201 positive samples were identified mainly with Luminex HLA-Typing method. Luminex HLA-Typing assay showed that only 53 cases were positives for the HLA-A*0201 allele. Among these cases, only 3 pairs were mismatched for the MiHAg HA-2. Acute GVHD occurred in 01 HA-2-mismatched pair while chronic GVHD was detected in 02 disparate couples. Univariate and multivariate analyses showed that MiHAg HA-2 disparity does not have any significant effect on the occurrence of either acute or chronic GVHD. This last one appeared to be correlated only with the age of patient (adulthood) (p: 0.011, OR: 22.092). Our findings support the previously reported data denying the influence of the HA-2 disparity on the GVHD occurrence after HSCT.
KW - Graft-vs-Host disease
KW - HA-2
KW - Haematopoietic Stem Cells Transplantation
KW - HLA-A0201
KW - Minor histocompatibility antigens
KW - Tunisian population
UR - http://www.scopus.com/inward/record.url?scp=77954975773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954975773&partnerID=8YFLogxK
U2 - 10.3109/08820131003775029
DO - 10.3109/08820131003775029
M3 - Article
C2 - 20653428
AN - SCOPUS:77954975773
VL - 39
SP - 611
EP - 620
JO - Immunological Investigations
JF - Immunological Investigations
SN - 0882-0139
IS - 6
ER -