| Original language | English |
|---|---|
| Pages (from-to) | 491-492 |
| Number of pages | 2 |
| Journal | Hepatobiliary and Pancreatic Diseases International |
| Volume | 18 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Oct 2019 |
ASJC Scopus subject areas
- Hepatology
- Gastroenterology
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Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas. / Covelli, Claudia; Parente, Paola; Pepe, Francesco; Pisapia, Pasquale; Fiordelisi, Fabiola; Malapelle, Umberto.
In: Hepatobiliary and Pancreatic Diseases International, Vol. 18, No. 5, 10.2019, p. 491-492.Research output: Contribution to journal › Letter › peer-review
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TY - JOUR
T1 - Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas
AU - Covelli, Claudia
AU - Parente, Paola
AU - Pepe, Francesco
AU - Pisapia, Pasquale
AU - Fiordelisi, Fabiola
AU - Malapelle, Umberto
N1 - Funding Information: All pancreatic tumors collected between January 1997 and August 2017 at Unit of Pathology of Foundation IRCCS ?Casa Sollievo della Sofferenza? were reviewed by two expert pathologists and four cases of SPN were selected. The median patient age was 40 years (range 23?46) with 3 females and 1 male. The median tumor size was 7?cm (range 4?15). Three tumors were located in the tail and one in the head of the pancreas. Morphological diagnosis was supported by IHC analysis. Representative formalin-fixed and paraffin-embedded (FFPE) tumor blocks and, in three cases, non-neoplastic tissue blocks were available. MMR proteins analysis (MLH1, PMS2, MSH2 and MSH6) was performed by IHC with the automated platform Autostainer Link 48 (DAKO, Carpinteria, CA, USA) according to the manufacturer's instructions. Briefly, 3-?m thick FFPE tissue sections were deparaffinized in xylene, rehydrated in graded alcohols, washed in double-distilled water and treated with DAKO solution (EnVision FLEX Target Retrieval Solution, High pH 50 ?) for antigen retrieval. The slides were treated with primary monoclonal antibodies against MLH1 (clone ES05, diluted 1:50, DAKO), PMS2 (clone EP51, diluted 1:40, DAKO), MSH2 (clone FE11, diluted 1:50, DAKO), and MSH6 (clone EP49, diluted 1:50, DAKO) at 97??C for 30?min. Antigen-antibody reaction was visualized using EnVision FLEX kit with diaminobenzidine as chromogen, slides were counterstained with hematoxylin and covered. In case of diffuse and homogeneous expression of all the four proteins, a proficient profile MMR (pMMR) was observed. Conversely, the loss of expression of one or more of these proteins indicated a dMMR profile. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/10
Y1 - 2019/10
UR - http://www.scopus.com/inward/record.url?scp=85073290217&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85073290217&partnerID=8YFLogxK
U2 - 10.1016/j.hbpd.2019.08.003
DO - 10.1016/j.hbpd.2019.08.003
M3 - Letter
C2 - 31477525
AN - SCOPUS:85073290217
VL - 18
SP - 491
EP - 492
JO - Hepatobiliary and Pancreatic Diseases International
JF - Hepatobiliary and Pancreatic Diseases International
SN - 1499-3872
IS - 5
ER -