Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas

Claudia Covelli; Paola Parente; Francesco Pepe; Pasquale Pisapia; Fabiola Fiordelisi; Umberto Malapelle

doi:10.1016/j.hbpd.2019.08.003

Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas

Claudia Covelli, Paola Parente, Francesco Pepe, Pasquale Pisapia, Fabiola Fiordelisi, Umberto Malapelle

IRCCS Casa Sollievo della Sofferenza

Research output: Contribution to journal › Letter › peer-review

Original language	English
Pages (from-to)	491-492
Number of pages	2
Journal	Hepatobiliary and Pancreatic Diseases International
Volume	18
Issue number	5
DOIs	https://doi.org/10.1016/j.hbpd.2019.08.003
Publication status	Published - Oct 2019

ASJC Scopus subject areas

Hepatology
Gastroenterology

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10.1016/j.hbpd.2019.08.003

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@article{93ba156ee4624ed6abb65e451bd7f377,

title = "Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas",

author = "Claudia Covelli and Paola Parente and Francesco Pepe and Pasquale Pisapia and Fabiola Fiordelisi and Umberto Malapelle",

note = "Funding Information: All pancreatic tumors collected between January 1997 and August 2017 at Unit of Pathology of Foundation IRCCS ?Casa Sollievo della Sofferenza? were reviewed by two expert pathologists and four cases of SPN were selected. The median patient age was 40 years (range 23?46) with 3 females and 1 male. The median tumor size was 7?cm (range 4?15). Three tumors were located in the tail and one in the head of the pancreas. Morphological diagnosis was supported by IHC analysis. Representative formalin-fixed and paraffin-embedded (FFPE) tumor blocks and, in three cases, non-neoplastic tissue blocks were available. MMR proteins analysis (MLH1, PMS2, MSH2 and MSH6) was performed by IHC with the automated platform Autostainer Link 48 (DAKO, Carpinteria, CA, USA) according to the manufacturer's instructions. Briefly, 3-?m thick FFPE tissue sections were deparaffinized in xylene, rehydrated in graded alcohols, washed in double-distilled water and treated with DAKO solution (EnVision FLEX Target Retrieval Solution, High pH 50 ?) for antigen retrieval. The slides were treated with primary monoclonal antibodies against MLH1 (clone ES05, diluted 1:50, DAKO), PMS2 (clone EP51, diluted 1:40, DAKO), MSH2 (clone FE11, diluted 1:50, DAKO), and MSH6 (clone EP49, diluted 1:50, DAKO) at 97??C for 30?min. Antigen-antibody reaction was visualized using EnVision FLEX kit with diaminobenzidine as chromogen, slides were counterstained with hematoxylin and covered. In case of diffuse and homogeneous expression of all the four proteins, a proficient profile MMR (pMMR) was observed. Conversely, the loss of expression of one or more of these proteins indicated a dMMR profile. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

year = "2019",

month = oct,

doi = "10.1016/j.hbpd.2019.08.003",

language = "English",

volume = "18",

pages = "491--492",

journal = "Hepatobiliary and Pancreatic Diseases International",

issn = "1499-3872",

publisher = "HBPD INT Press",

number = "5",

}

TY - JOUR

T1 - Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas

AU - Covelli, Claudia

AU - Parente, Paola

AU - Pepe, Francesco

AU - Pisapia, Pasquale

AU - Fiordelisi, Fabiola

AU - Malapelle, Umberto

N1 - Funding Information: All pancreatic tumors collected between January 1997 and August 2017 at Unit of Pathology of Foundation IRCCS ?Casa Sollievo della Sofferenza? were reviewed by two expert pathologists and four cases of SPN were selected. The median patient age was 40 years (range 23?46) with 3 females and 1 male. The median tumor size was 7?cm (range 4?15). Three tumors were located in the tail and one in the head of the pancreas. Morphological diagnosis was supported by IHC analysis. Representative formalin-fixed and paraffin-embedded (FFPE) tumor blocks and, in three cases, non-neoplastic tissue blocks were available. MMR proteins analysis (MLH1, PMS2, MSH2 and MSH6) was performed by IHC with the automated platform Autostainer Link 48 (DAKO, Carpinteria, CA, USA) according to the manufacturer's instructions. Briefly, 3-?m thick FFPE tissue sections were deparaffinized in xylene, rehydrated in graded alcohols, washed in double-distilled water and treated with DAKO solution (EnVision FLEX Target Retrieval Solution, High pH 50 ?) for antigen retrieval. The slides were treated with primary monoclonal antibodies against MLH1 (clone ES05, diluted 1:50, DAKO), PMS2 (clone EP51, diluted 1:40, DAKO), MSH2 (clone FE11, diluted 1:50, DAKO), and MSH6 (clone EP49, diluted 1:50, DAKO) at 97??C for 30?min. Antigen-antibody reaction was visualized using EnVision FLEX kit with diaminobenzidine as chromogen, slides were counterstained with hematoxylin and covered. In case of diffuse and homogeneous expression of all the four proteins, a proficient profile MMR (pMMR) was observed. Conversely, the loss of expression of one or more of these proteins indicated a dMMR profile. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/10

Y1 - 2019/10

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U2 - 10.1016/j.hbpd.2019.08.003

DO - 10.1016/j.hbpd.2019.08.003

M3 - Letter

C2 - 31477525

AN - SCOPUS:85073290217

VL - 18

SP - 491

EP - 492

JO - Hepatobiliary and Pancreatic Diseases International

JF - Hepatobiliary and Pancreatic Diseases International

SN - 1499-3872

IS - 5

ER -

Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas

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