Mitochondrial aspartate aminotransferase isoenzyme

A biochemical marker for the clinical management of alcoholics?

Teodora Macchia, Rosanna Mancinelli, Stefano Gentili, Mauro Ceccanti, Rita Devito, Maria L. Attilia, Franco Taggi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Serum mitochondrial and total aspartate aminotransferase activity was quantified by a characterized immunochemical method in 126 subjects, 44 healthy controls and 82 chronic alcoholics (51 outpatients and 31 monitored through 15 days). The monitored alcoholics were divided into actual abstinents (n = 21) and drinkers (n = 10) by blood ethanol concentration performed daily. The aims of the present study were: (a) to compare the diagnostic usefulness of the mitochondrial isoenzyme and the mitochondrial/total aspartate aminotransferase ratio to detect problematic drinkers; (b) to evaluate the suitability of these indices to monitor abstinence, a difficulty not yet solved in the clinical management of alcoholics. The results demonstrated the mitochondrial isoenzyme to be more suitable to discriminate between controls and alcoholics (Kruskal and Wallis ANOVA, Bonferroni test, P <10-5) and mostly between actual drinkers and other alcoholics (P <0.041). So acute alcohol consumption may be a significant, suggestive and until now inadequately examined factor in evaluating the suitability of mAST as a marker. The results, showing that mAST peaks quickly appear in the presence of a new alcohol intake, should indicate mAST as a possible marker of acute alcohol intake useful in checking self-claimed abstinence.

Original languageEnglish
Pages (from-to)79-96
Number of pages18
JournalClinica Chimica Acta
Volume263
Issue number1
DOIs
Publication statusPublished - Jul 4 1997

Fingerprint

Mitochondrial Aspartate Aminotransferase
Alcoholics
Isoenzymes
Biomarkers
Alcohols
Aspartate Aminotransferases
Analysis of variance (ANOVA)
Blood
Ethanol
Alcohol Drinking
Analysis of Variance
Healthy Volunteers
Outpatients
Serum

Keywords

  • Alcohol abuse and abstinence markers
  • Aspartate aminotransferase isoenzyme
  • Blood alcohol concentration

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Mitochondrial aspartate aminotransferase isoenzyme : A biochemical marker for the clinical management of alcoholics? / Macchia, Teodora; Mancinelli, Rosanna; Gentili, Stefano; Ceccanti, Mauro; Devito, Rita; Attilia, Maria L.; Taggi, Franco.

In: Clinica Chimica Acta, Vol. 263, No. 1, 04.07.1997, p. 79-96.

Research output: Contribution to journalArticle

Macchia, Teodora ; Mancinelli, Rosanna ; Gentili, Stefano ; Ceccanti, Mauro ; Devito, Rita ; Attilia, Maria L. ; Taggi, Franco. / Mitochondrial aspartate aminotransferase isoenzyme : A biochemical marker for the clinical management of alcoholics?. In: Clinica Chimica Acta. 1997 ; Vol. 263, No. 1. pp. 79-96.
@article{e5c7201016d643b0b486866c2f586448,
title = "Mitochondrial aspartate aminotransferase isoenzyme: A biochemical marker for the clinical management of alcoholics?",
abstract = "Serum mitochondrial and total aspartate aminotransferase activity was quantified by a characterized immunochemical method in 126 subjects, 44 healthy controls and 82 chronic alcoholics (51 outpatients and 31 monitored through 15 days). The monitored alcoholics were divided into actual abstinents (n = 21) and drinkers (n = 10) by blood ethanol concentration performed daily. The aims of the present study were: (a) to compare the diagnostic usefulness of the mitochondrial isoenzyme and the mitochondrial/total aspartate aminotransferase ratio to detect problematic drinkers; (b) to evaluate the suitability of these indices to monitor abstinence, a difficulty not yet solved in the clinical management of alcoholics. The results demonstrated the mitochondrial isoenzyme to be more suitable to discriminate between controls and alcoholics (Kruskal and Wallis ANOVA, Bonferroni test, P <10-5) and mostly between actual drinkers and other alcoholics (P <0.041). So acute alcohol consumption may be a significant, suggestive and until now inadequately examined factor in evaluating the suitability of mAST as a marker. The results, showing that mAST peaks quickly appear in the presence of a new alcohol intake, should indicate mAST as a possible marker of acute alcohol intake useful in checking self-claimed abstinence.",
keywords = "Alcohol abuse and abstinence markers, Aspartate aminotransferase isoenzyme, Blood alcohol concentration",
author = "Teodora Macchia and Rosanna Mancinelli and Stefano Gentili and Mauro Ceccanti and Rita Devito and Attilia, {Maria L.} and Franco Taggi",
year = "1997",
month = "7",
day = "4",
doi = "10.1016/S0009-8981(97)00046-6",
language = "English",
volume = "263",
pages = "79--96",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Mitochondrial aspartate aminotransferase isoenzyme

T2 - A biochemical marker for the clinical management of alcoholics?

AU - Macchia, Teodora

AU - Mancinelli, Rosanna

AU - Gentili, Stefano

AU - Ceccanti, Mauro

AU - Devito, Rita

AU - Attilia, Maria L.

AU - Taggi, Franco

PY - 1997/7/4

Y1 - 1997/7/4

N2 - Serum mitochondrial and total aspartate aminotransferase activity was quantified by a characterized immunochemical method in 126 subjects, 44 healthy controls and 82 chronic alcoholics (51 outpatients and 31 monitored through 15 days). The monitored alcoholics were divided into actual abstinents (n = 21) and drinkers (n = 10) by blood ethanol concentration performed daily. The aims of the present study were: (a) to compare the diagnostic usefulness of the mitochondrial isoenzyme and the mitochondrial/total aspartate aminotransferase ratio to detect problematic drinkers; (b) to evaluate the suitability of these indices to monitor abstinence, a difficulty not yet solved in the clinical management of alcoholics. The results demonstrated the mitochondrial isoenzyme to be more suitable to discriminate between controls and alcoholics (Kruskal and Wallis ANOVA, Bonferroni test, P <10-5) and mostly between actual drinkers and other alcoholics (P <0.041). So acute alcohol consumption may be a significant, suggestive and until now inadequately examined factor in evaluating the suitability of mAST as a marker. The results, showing that mAST peaks quickly appear in the presence of a new alcohol intake, should indicate mAST as a possible marker of acute alcohol intake useful in checking self-claimed abstinence.

AB - Serum mitochondrial and total aspartate aminotransferase activity was quantified by a characterized immunochemical method in 126 subjects, 44 healthy controls and 82 chronic alcoholics (51 outpatients and 31 monitored through 15 days). The monitored alcoholics were divided into actual abstinents (n = 21) and drinkers (n = 10) by blood ethanol concentration performed daily. The aims of the present study were: (a) to compare the diagnostic usefulness of the mitochondrial isoenzyme and the mitochondrial/total aspartate aminotransferase ratio to detect problematic drinkers; (b) to evaluate the suitability of these indices to monitor abstinence, a difficulty not yet solved in the clinical management of alcoholics. The results demonstrated the mitochondrial isoenzyme to be more suitable to discriminate between controls and alcoholics (Kruskal and Wallis ANOVA, Bonferroni test, P <10-5) and mostly between actual drinkers and other alcoholics (P <0.041). So acute alcohol consumption may be a significant, suggestive and until now inadequately examined factor in evaluating the suitability of mAST as a marker. The results, showing that mAST peaks quickly appear in the presence of a new alcohol intake, should indicate mAST as a possible marker of acute alcohol intake useful in checking self-claimed abstinence.

KW - Alcohol abuse and abstinence markers

KW - Aspartate aminotransferase isoenzyme

KW - Blood alcohol concentration

UR - http://www.scopus.com/inward/record.url?scp=0030747634&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030747634&partnerID=8YFLogxK

U2 - 10.1016/S0009-8981(97)00046-6

DO - 10.1016/S0009-8981(97)00046-6

M3 - Article

VL - 263

SP - 79

EP - 96

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1

ER -