Mitochondrial changes in platelets are not related to those in skeletal muscle during human septic shock

Alessandro Protti, Francesco Fortunato, Maria L. Caspani, Mauro Pluderi, Valeria Lucchini, Nadia Grimoldi, Luigi P. Solimeno, Gigliola Fagiolari, Patrizia Ciscato, Samis M A Zella, Maurizio Moggio, Giacomo P. Comi, Luciano Gattinoni

Research output: Contribution to journalArticle

Abstract

Platelets can serve as general markers of mitochondrial (dys)function during several human diseases. Whether this holds true even during sepsis is unknown. Using spectrophotometry, we measured mitochondrial respiratory chain biochemistry in platelets and triceps brachii muscle of thirty patients with septic shock (within 24 hours from admission to Intensive Care) and ten surgical controls (during surgery). Results were expressed relative to citrate synthase (CS) activity, a marker of mitochondrial density. Patients with septic shock had lower nicotinamide adenine dinucleotide dehydrogenase (NADH)/CS (p = 0.015), complex I/CS (p = 0.018), complex I and III/CS (p2 = 0.00; p = 0.683) complex I/CS (r2 = 0.05; p = 0.173), complex I and III/CS (r2 = 0.01; p = 0.485), succinate dehydrogenase (SDH)/CS (r 2 = 0.00; p = 0.884), complex II and III/CS (r2 = 0.00; p = 0.927) and complex IV/CS (r2 = 0.00; p = 0.906) activities in platelets were not associated with those in triceps brachii muscle. In conclusion, several respiratory chain enzymes were variably inhibited in platelets, but not in triceps brachii muscle, of patients with septic shock. Sepsis-induced mitochondrial changes in platelets do not reflect those in other organs.

Original languageEnglish
Article numbere96205
JournalPLoS One
Volume9
Issue number5
DOIs
Publication statusPublished - May 1 2014

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Citrate (si)-Synthase
septic shock
citrate (si)-synthase
Septic Shock
Platelets
Muscle
skeletal muscle
Skeletal Muscle
Blood Platelets
NADH dehydrogenase (ubiquinone)
electron transport chain
Electron Transport
Muscles
muscles
Sepsis
sepsis (infection)
Biochemistry
Succinate Dehydrogenase
NAD (coenzyme)
Spectrophotometry

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Mitochondrial changes in platelets are not related to those in skeletal muscle during human septic shock. / Protti, Alessandro; Fortunato, Francesco; Caspani, Maria L.; Pluderi, Mauro; Lucchini, Valeria; Grimoldi, Nadia; Solimeno, Luigi P.; Fagiolari, Gigliola; Ciscato, Patrizia; Zella, Samis M A; Moggio, Maurizio; Comi, Giacomo P.; Gattinoni, Luciano.

In: PLoS One, Vol. 9, No. 5, e96205, 01.05.2014.

Research output: Contribution to journalArticle

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AU - Lucchini, Valeria

AU - Grimoldi, Nadia

AU - Solimeno, Luigi P.

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AU - Ciscato, Patrizia

AU - Zella, Samis M A

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