Mitochondrial-dependent Autoimmunity in Membranous Nephropathy of IgG4-related Disease

Simona Buelli, Luca Perico, Miriam Galbusera, Mauro Abbate, Marina Morigi, Rubina Novelli, Elena Gagliardini, Chiara Tentori, Daniela Rottoli, Ettore Sabadini, Takao Saito, Mitsuhiro Kawano, Takako Saeki, Carlamaria Zoja, Giuseppe Remuzzi, Ariela Benigni

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The pathophysiology of glomerular lesions of membranous nephropathy (MN), including seldom-reported IgG4-related disease, is still elusive. Unlike in idiopathic MN where IgG4 prevails, in this patient IgG3 was predominant in glomerular deposits in the absence of circulating anti-phospholipase A2 receptor antibodies, suggesting a distinct pathologic process. Here we documented that IgG4 retrieved from the serum of our propositus reacted against carbonic anhydrase II (CAII) at the podocyte surface. In patient's biopsy, glomerular CAII staining increased and co-localized with subepithelial IgG4 deposits along the capillary walls. Patient's IgG4 caused a drop in cell pH followed by mitochondrial dysfunction, excessive ROS production and cytoskeletal reorganization in cultured podocytes. These events promoted mitochondrial superoxide-dismutase-2 (SOD2) externalization on the plasma membrane, becoming recognizable by complement-binding IgG3 anti-SOD2. Among patients with IgG4-related disease only sera of those with IgG4 anti-CAII antibodies caused low intracellular pH and mitochondrial alterations underlying SOD2 externalization. Circulating IgG4 anti-CAII can cause podocyte injury through processes of intracellular acidification, mitochondrial oxidative stress and neoantigen induction in patients with IgG4 related disease. The onset of MN in a subset of patients could be due to IgG4 antibodies recognizing CAII with consequent exposure of mitochondrial neoantigen in the context of multifactorial pathogenesis of disease.

Original languageEnglish
Pages (from-to)456-466
Number of pages11
JournalEBioMedicine
Volume2
Issue number5
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Membranous Glomerulonephritis
Autoimmunity
Immunoglobulin G
Carbonic Anhydrase II
Podocytes
Phospholipase A2 Receptors
Antibodies
Deposits
Oxidative stress
Biopsy
Acidification
Pathologic Processes
Cell membranes
Serum
Oxidative Stress

Keywords

  • Carbonic anhydrase II
  • IgG4-related disease
  • Membranous nephropathy
  • Podocyte
  • Superoxide dismutase 2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mitochondrial-dependent Autoimmunity in Membranous Nephropathy of IgG4-related Disease. / Buelli, Simona; Perico, Luca; Galbusera, Miriam; Abbate, Mauro; Morigi, Marina; Novelli, Rubina; Gagliardini, Elena; Tentori, Chiara; Rottoli, Daniela; Sabadini, Ettore; Saito, Takao; Kawano, Mitsuhiro; Saeki, Takako; Zoja, Carlamaria; Remuzzi, Giuseppe; Benigni, Ariela.

In: EBioMedicine, Vol. 2, No. 5, 01.05.2015, p. 456-466.

Research output: Contribution to journalArticle

Buelli, S, Perico, L, Galbusera, M, Abbate, M, Morigi, M, Novelli, R, Gagliardini, E, Tentori, C, Rottoli, D, Sabadini, E, Saito, T, Kawano, M, Saeki, T, Zoja, C, Remuzzi, G & Benigni, A 2015, 'Mitochondrial-dependent Autoimmunity in Membranous Nephropathy of IgG4-related Disease', EBioMedicine, vol. 2, no. 5, pp. 456-466. https://doi.org/10.1016/j.ebiom.2015.03.003
Buelli, Simona ; Perico, Luca ; Galbusera, Miriam ; Abbate, Mauro ; Morigi, Marina ; Novelli, Rubina ; Gagliardini, Elena ; Tentori, Chiara ; Rottoli, Daniela ; Sabadini, Ettore ; Saito, Takao ; Kawano, Mitsuhiro ; Saeki, Takako ; Zoja, Carlamaria ; Remuzzi, Giuseppe ; Benigni, Ariela. / Mitochondrial-dependent Autoimmunity in Membranous Nephropathy of IgG4-related Disease. In: EBioMedicine. 2015 ; Vol. 2, No. 5. pp. 456-466.
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AU - Morigi, Marina

AU - Novelli, Rubina

AU - Gagliardini, Elena

AU - Tentori, Chiara

AU - Rottoli, Daniela

AU - Sabadini, Ettore

AU - Saito, Takao

AU - Kawano, Mitsuhiro

AU - Saeki, Takako

AU - Zoja, Carlamaria

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N2 - The pathophysiology of glomerular lesions of membranous nephropathy (MN), including seldom-reported IgG4-related disease, is still elusive. Unlike in idiopathic MN where IgG4 prevails, in this patient IgG3 was predominant in glomerular deposits in the absence of circulating anti-phospholipase A2 receptor antibodies, suggesting a distinct pathologic process. Here we documented that IgG4 retrieved from the serum of our propositus reacted against carbonic anhydrase II (CAII) at the podocyte surface. In patient's biopsy, glomerular CAII staining increased and co-localized with subepithelial IgG4 deposits along the capillary walls. Patient's IgG4 caused a drop in cell pH followed by mitochondrial dysfunction, excessive ROS production and cytoskeletal reorganization in cultured podocytes. These events promoted mitochondrial superoxide-dismutase-2 (SOD2) externalization on the plasma membrane, becoming recognizable by complement-binding IgG3 anti-SOD2. Among patients with IgG4-related disease only sera of those with IgG4 anti-CAII antibodies caused low intracellular pH and mitochondrial alterations underlying SOD2 externalization. Circulating IgG4 anti-CAII can cause podocyte injury through processes of intracellular acidification, mitochondrial oxidative stress and neoantigen induction in patients with IgG4 related disease. The onset of MN in a subset of patients could be due to IgG4 antibodies recognizing CAII with consequent exposure of mitochondrial neoantigen in the context of multifactorial pathogenesis of disease.

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