Mitochondrial DNA mutations in patients with postlingual, nonsyndromic hearing impairment

Howard T. Jacobs, Timothy P. Hutchin, Timo Käppi, Greta Gillies, Kia Minkkinen, John Walker, Karen Thompson, Anja T. Rovio, Massimo Carella, Salvatore Melchionda, Leopoldo Zelante, Paolo Gasparini, Ilmari Pyykkö, Zahid H. Shah, Massimo Zeviani, Robert F. Mueller

Research output: Contribution to journalArticlepeer-review


Mitochondrial mutations have previously been reported anecdotally in families with maternally inherited, nonsyndromic hearing impairment. To ascertain the contribution of mitochondrial mutations to postlingual but early-onset, nonsyndromic hearing impairment, we screened patients collected from within two different populations (southern Italy and UK) for previously reported mtDNA mutations associated with hearing disorders. Primer extension (SNP analysis) was used to screen for specific mutations, revealing cases of heteroplasmy and its extent. The most frequently implicated tRNA genes, Leu(UUR) and Ser(UCN), were also sequenced in all Italian patients. All tRNA genes were sequenced in those UK patients showing the clearest likelihood of maternal inheritance. Causative mtDNA mutations were found in approximately 5% of patients in both populations, representing almost 10% of cases that were clearly familial. Age of onset, where known, was generally before adulthood, and hearing loss was typically progressive. Haplogroup analysis revealed a possible excess of haplogroup cluster HV in the patients, compared with population controls, but of borderline statistical significance. In contrast, we did not find any of the previously reported mtDNA mutations, nor a significant deviation from haplogroup cluster frequencies typical of the control population, in patients with late adult-onset hearing loss (age-related hearing impairment) from the UK or Finland.

Original languageEnglish
Pages (from-to)26-33
Number of pages8
JournalEuropean Journal of Human Genetics
Issue number1
Publication statusPublished - Jan 2005


  • Aminoglycoside
  • Deafness
  • Haplogroup
  • Mitochondrial disease
  • Ribosomal RNA
  • Transfer RNA

ASJC Scopus subject areas

  • Genetics(clinical)


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