Mitochondrial dysfunction in Parkinson disease: Evidence in mutant PARK2 fibroblasts

Maria C. Zanellati, Valentina Monti, Chiara Barzaghi, Chiara Reale, Nardo Nardocci, Alberto Albanese, Enza M. Valente, Daniele Ghezzi, Barbara Garavaglia

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Mutations in PARK2, encoding Parkin, cause an autosomal recessive form of juvenile Parkinson Disease (JPD). The aim of the present study was to investigate the impact of PARK2 mutations on mitochondrial function and morphology in human skin fibroblasts. We analyzed cells obtained from four patients clinically characterized by JPD, harboring recessive mutations in PARK2. By quantitative PCR we found a reduction (m), confirmed by JC-1 analysis. The mitochondrial network was comparable between mutant and control cells but, interestingly, a "chain-like" network was found only in mutant fibroblasts. Dissipation of ΔΨm usually leads to mitochondrial fragmentation in healthy cells and eventually to mitophagy; however, this behavior was not observed in patients' fibroblasts. The absence of mitochondrial fragmentation in mutant Parkin fibroblasts could results in accumulation of damaged mitochondria not targeted to mitophagy. This condition should increase the oxidative stress and lead to cellular dysfunction and death. Our results suggest that PARK2 mutations cause mitochondrial impairment, in particular reduction in ATP cellular levels and alteration of ΔΨm, even in non-neuronal cells and confirm the hypothesis that Parkin holds a pivotal role in pro-fission events.

Original languageEnglish
Article number078
JournalFrontiers in Genetics
Volume6
Issue numberMAR
DOIs
Publication statusPublished - 2015

Fingerprint

Parkinson Disease
Mitochondrial Degradation
Fibroblasts
Mutation
Parkinsonian Disorders
Mitochondria
Oxidative Stress
Adenosine Triphosphate
Polymerase Chain Reaction
Skin

Keywords

  • Mitochondrial dynamics
  • Mitochondrial membrane potential
  • Oxygen consumption
  • PARK2
  • Parkin

ASJC Scopus subject areas

  • Genetics
  • Molecular Medicine
  • Genetics(clinical)

Cite this

Mitochondrial dysfunction in Parkinson disease : Evidence in mutant PARK2 fibroblasts. / Zanellati, Maria C.; Monti, Valentina; Barzaghi, Chiara; Reale, Chiara; Nardocci, Nardo; Albanese, Alberto; Valente, Enza M.; Ghezzi, Daniele; Garavaglia, Barbara.

In: Frontiers in Genetics, Vol. 6, No. MAR, 078, 2015.

Research output: Contribution to journalArticle

Zanellati, Maria C. ; Monti, Valentina ; Barzaghi, Chiara ; Reale, Chiara ; Nardocci, Nardo ; Albanese, Alberto ; Valente, Enza M. ; Ghezzi, Daniele ; Garavaglia, Barbara. / Mitochondrial dysfunction in Parkinson disease : Evidence in mutant PARK2 fibroblasts. In: Frontiers in Genetics. 2015 ; Vol. 6, No. MAR.
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