Mitochondrial G protein coupled receptor kinase 2 regulates proinflammatory responses in macrophages

D. Sorriento, A. Fusco, M. Ciccarelli, A. Rungi, A. Anastasio, A. Carillo, G. W. Dorn, B. Trimarco, G. Iaccarino

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

G-protein-coupled receptor kinase 2 (GRK2) levels are elevated in inflammation but its role is not clear yet. Here we show that GRK2 expression is dependent on NFκB transcriptional activity. In macrophages, LPS induces GRK2 accumulation in mitochondria increasing biogenesis. The overexpression of the carboxy-terminal domain of GRK2 (βARK-ct), known to displace GRK2 from plasma membranes, induces earlier localization of GRK2 to mitochondria in response to LPS leading to increased mt-DNA transcription and reduced ROS production and cytokine expression. Our study shows the relevance of GRK2 subcellular localization in macrophage biology and its potential therapeutic properties in inflammation.

Original languageEnglish
Pages (from-to)3487-3494
Number of pages8
JournalFEBS Letters
Volume587
Issue number21
DOIs
Publication statusPublished - Nov 1 2013

Fingerprint

G-Protein-Coupled Receptor Kinase 2
Macrophages
Mitochondrial Proteins
Mitochondria
Inflammation
Transcription
Cell membranes
Cell Membrane
Cytokines
DNA

Keywords

  • βARK-ct
  • GRK2
  • Inflammation
  • Mitochondria
  • Subcellular localization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Sorriento, D., Fusco, A., Ciccarelli, M., Rungi, A., Anastasio, A., Carillo, A., ... Iaccarino, G. (2013). Mitochondrial G protein coupled receptor kinase 2 regulates proinflammatory responses in macrophages. FEBS Letters, 587(21), 3487-3494. https://doi.org/10.1016/j.febslet.2013.09.002

Mitochondrial G protein coupled receptor kinase 2 regulates proinflammatory responses in macrophages. / Sorriento, D.; Fusco, A.; Ciccarelli, M.; Rungi, A.; Anastasio, A.; Carillo, A.; Dorn, G. W.; Trimarco, B.; Iaccarino, G.

In: FEBS Letters, Vol. 587, No. 21, 01.11.2013, p. 3487-3494.

Research output: Contribution to journalArticle

Sorriento, D, Fusco, A, Ciccarelli, M, Rungi, A, Anastasio, A, Carillo, A, Dorn, GW, Trimarco, B & Iaccarino, G 2013, 'Mitochondrial G protein coupled receptor kinase 2 regulates proinflammatory responses in macrophages', FEBS Letters, vol. 587, no. 21, pp. 3487-3494. https://doi.org/10.1016/j.febslet.2013.09.002
Sorriento D, Fusco A, Ciccarelli M, Rungi A, Anastasio A, Carillo A et al. Mitochondrial G protein coupled receptor kinase 2 regulates proinflammatory responses in macrophages. FEBS Letters. 2013 Nov 1;587(21):3487-3494. https://doi.org/10.1016/j.febslet.2013.09.002
Sorriento, D. ; Fusco, A. ; Ciccarelli, M. ; Rungi, A. ; Anastasio, A. ; Carillo, A. ; Dorn, G. W. ; Trimarco, B. ; Iaccarino, G. / Mitochondrial G protein coupled receptor kinase 2 regulates proinflammatory responses in macrophages. In: FEBS Letters. 2013 ; Vol. 587, No. 21. pp. 3487-3494.
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