Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: Analysis at the single cell and single organelle level

Stefano Salvioli, Jurek Dobrucki, Laura Moretti, Leonarda Troiano, Maria Garcia Fernandez, Marcello Pinti, Jessica Pedrazzi, Claudio Franceschi, Andrea Cossarizza

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Apoptosis is a complex phenomenon during which several events occur. A growing interest exists on the role and functionality of mitochondria during this type of cell death. The responsibility of modifications in mitochondrial membrane potential (ΔΨ) in triggering apoptosis is under investigation. Methods: We evaluated ΔΨ changes in HL60 cells treated with staurosporine (STS). Flow cytometry and confocal microscopy have been used to analyze samples stained with two ΔΨ-sensitive probes, JC-1 and MitoTracker(TM) Red CMXRos. Results: At the cellular level, we found heterogeneic behavior. Indeed, after STS treatment, some cells displayed typical markers of apoptosis and a collapse in ΔΨ. Others were apoptotic with no changes in ΔΨ, others changed ΔΨ without being apoptotic, and others were healthy. The same heterogeneic response to STS was found at the single organelle level. In a given cell, some mitochondria were depolarized whereas others were not. Conclusion: In this model of apoptosis, changes in ΔΨ can be different among cells of the same type and among different organelles of the same cell. The collapse in ΔΨ is thus a heterogeneic phenomenon that seems to be an ancillary event following the irreversible phase of the apoptotic process. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)189-197
Number of pages9
JournalCytometry
Volume40
Issue number3
DOIs
Publication statusPublished - 2000

Keywords

  • Apoptosis
  • Confocal microscopy
  • Flow cytometry
  • HL60 cells
  • JC- 1
  • Mitochondria
  • Mitochondrial membrane potential
  • MitoTracker

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

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