Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach

Roberta Ferraresi; Leonarda Troiano; Daniela Rossi; Elena Gualdi; Enrico Lugli; Cristina Mussini; Andrea Cossarizza

doi:10.1016/j.mito.2004.05.015

Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach

Roberta Ferraresi, Leonarda Troiano, Daniela Rossi, Elena Gualdi, Enrico Lugli, Cristina Mussini, Andrea Cossarizza

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.

Original language	English
Pages (from-to)	271-278
Number of pages	8
Journal	Mitochondrion
Volume	4
Issue number	2-3
DOIs	https://doi.org/10.1016/j.mito.2004.05.015
Publication status	Published - Jul 2004

Keywords

Flow cytometry
HAART
Mitochondria

ASJC Scopus subject areas

Biophysics

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10.1016/j.mito.2004.05.015

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abstract = "Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.",

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T2 - A cytometric approach

AU - Ferraresi, Roberta

AU - Troiano, Leonarda

AU - Rossi, Daniela

AU - Gualdi, Elena

AU - Lugli, Enrico

AU - Mussini, Cristina

AU - Cossarizza, Andrea

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AB - Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.

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Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach

Abstract

Keywords

ASJC Scopus subject areas

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