Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach

Roberta Ferraresi; Leonarda Troiano; Daniela Rossi; Elena Gualdi; Enrico Lugli; Cristina Mussini; Andrea Cossarizza

doi:10.1016/j.mito.2004.05.015

Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach

Roberta Ferraresi, Leonarda Troiano, Daniela Rossi, Elena Gualdi, Enrico Lugli, Cristina Mussini, Andrea Cossarizza

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.

Original language	English
Pages (from-to)	271-278
Number of pages	8
Journal	Mitochondrion
Volume	4
Issue number	2-3
DOIs	https://doi.org/10.1016/j.mito.2004.05.015
Publication status	Published - Jul 2004

Keywords

Flow cytometry
HAART
Mitochondria

ASJC Scopus subject areas

Biophysics

Access to Document

10.1016/j.mito.2004.05.015

Fingerprint Dive into the research topics of 'Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach'. Together they form a unique fingerprint.

Cite this

@article{18b42e9ff5444b84acf16a32734d9339,

title = "Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach",

abstract = "Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.",

keywords = "Flow cytometry, HAART, Mitochondria",

author = "Roberta Ferraresi and Leonarda Troiano and Daniela Rossi and Elena Gualdi and Enrico Lugli and Cristina Mussini and Andrea Cossarizza",

year = "2004",

month = jul,

doi = "10.1016/j.mito.2004.05.015",

language = "English",

volume = "4",

pages = "271--278",

journal = "Mitochondrion",

issn = "1567-7249",

publisher = "Elsevier",

number = "2-3",

}

TY - JOUR

T1 - Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase

T2 - A cytometric approach

AU - Ferraresi, Roberta

AU - Troiano, Leonarda

AU - Rossi, Daniela

AU - Gualdi, Elena

AU - Lugli, Enrico

AU - Mussini, Cristina

AU - Cossarizza, Andrea

PY - 2004/7

Y1 - 2004/7

N2 - Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.

AB - Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.

KW - Flow cytometry

KW - HAART

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=9244223649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9244223649&partnerID=8YFLogxK

U2 - 10.1016/j.mito.2004.05.015

DO - 10.1016/j.mito.2004.05.015

M3 - Article

C2 - 16120391

AN - SCOPUS:9244223649

VL - 4

SP - 271

EP - 278

JO - Mitochondrion

JF - Mitochondrion

SN - 1567-7249

IS - 2-3

ER -

Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this