Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach

Roberta Ferraresi, Leonarda Troiano, Daniela Rossi, Elena Gualdi, Enrico Lugli, Cristina Mussini, Andrea Cossarizza

Research output: Contribution to journalArticlepeer-review

Abstract

Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalMitochondrion
Volume4
Issue number2-3
DOIs
Publication statusPublished - Jul 2004

Keywords

  • Flow cytometry
  • HAART
  • Mitochondria

ASJC Scopus subject areas

  • Biophysics

Fingerprint Dive into the research topics of 'Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: A cytometric approach'. Together they form a unique fingerprint.

Cite this