TY - JOUR
T1 - Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase
T2 - A cytometric approach
AU - Ferraresi, Roberta
AU - Troiano, Leonarda
AU - Rossi, Daniela
AU - Gualdi, Elena
AU - Lugli, Enrico
AU - Mussini, Cristina
AU - Cossarizza, Andrea
PY - 2004/7
Y1 - 2004/7
N2 - Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.
AB - Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.
KW - Flow cytometry
KW - HAART
KW - Mitochondria
UR - http://www.scopus.com/inward/record.url?scp=9244223649&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9244223649&partnerID=8YFLogxK
U2 - 10.1016/j.mito.2004.05.015
DO - 10.1016/j.mito.2004.05.015
M3 - Article
C2 - 16120391
AN - SCOPUS:9244223649
VL - 4
SP - 271
EP - 278
JO - Mitochondrion
JF - Mitochondrion
SN - 1567-7249
IS - 2-3
ER -