Mitochondrial oxidative metabolism in motor neuron degeneration (mnd) mouse central nervous system

M. Bertamini, B. Marzani, R. Guarneri, P. Guarneri, P. Bigini, T. Mennini, Daniela Curti

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The mnd mouse spontaneously develops slowly evolving motoneuron pathology leading to progressive motor impairment. There is strong evidence that a complex interplay between oxidative stress, mitochondria abnormalities and alteration of glutamate neurotransmission plays an important role in the pathogenesis of motor neuron diseases. Therefore, we investigated the presence of mitochondrial dysfunction in frontal, central (comprising the motor area) and occipital regions of the cerebral cortex and in the spinal cord of 35-week-old mnd mice. Lipid peroxide derivatives reacting with thiobarbituric acid (TBARS) were measured in the cervical, thoracic and lumbar spinal cord. In addition biochemical and behavioural analyses were carried out in mnd mice chronically treated with L-carnitine from the 11th to the 34th week of life (mndT mice). Slight but significant alterations of mitochondrial enzyme activities were seen in the mnd cortical regions. The central area was the most affected and both (complex I, IV and citrate synthase were decreased with respect to controls. The rate of oxygen consumption (QO2) was markedly decreased in both the upper (cervical + upper portion of the thoracic region) and lower (lumbar + lower portion of the thoracic region) mnd spinal cord. The level of TBARS showed a rostro-caudal trend to increase, being 30% higher in the lumbar tract of mnd mice in comparison with controls. L-carnitine treatment increased the mitochondrial enzyme activities in cortical regions towards control value and was effective in enhancing QO2 and decreasing TBARS levels in the spinal cord of mndT. Behavioural testing showed that L-carnitine significantly delayed the onset of motor behaviour impairment. This beneficial effect was declining at 35 week of age, when the biochemical measurements were performed.

Original languageEnglish
Pages (from-to)2291-2296
Number of pages6
JournalEuropean Journal of Neuroscience
Volume16
Issue number12
DOIs
Publication statusPublished - 2002

Fingerprint

Nerve Degeneration
Motor Neurons
Central Nervous System
Spinal Cord
Carnitine
Thorax
Lumbosacral Region
Citrate (si)-Synthase
Occipital Lobe
Motor Neuron Disease
Lipid Peroxides
Motor Cortex
Enzymes
Oxygen Consumption
Synaptic Transmission
Cerebral Cortex
Glutamic Acid
Mitochondria
Oxidative Stress
Pathology

Keywords

  • Behaviour
  • L-carnitine
  • Lipid peroxidation
  • Motoneuron disease

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mitochondrial oxidative metabolism in motor neuron degeneration (mnd) mouse central nervous system. / Bertamini, M.; Marzani, B.; Guarneri, R.; Guarneri, P.; Bigini, P.; Mennini, T.; Curti, Daniela.

In: European Journal of Neuroscience, Vol. 16, No. 12, 2002, p. 2291-2296.

Research output: Contribution to journalArticle

Bertamini, M. ; Marzani, B. ; Guarneri, R. ; Guarneri, P. ; Bigini, P. ; Mennini, T. ; Curti, Daniela. / Mitochondrial oxidative metabolism in motor neuron degeneration (mnd) mouse central nervous system. In: European Journal of Neuroscience. 2002 ; Vol. 16, No. 12. pp. 2291-2296.
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