MK801 attenuates secondary injury in a mouse experimental compression model of spinal cord trauma

Emanuela Esposito, Irene Paterniti, Emanuela Mazzon, Tiziana Genovese, Maria Galuppo, Rosaria Meli, Placido Bramanti, Salvatore Cuzzocrea

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Glutamergic excitotoxicity has been shown to play a deleterious role in the pathophysiology of spinal cord injury (SCI). The aim of this study was to investigate the neuroprotective effect of dizocilpine maleate, MK801 (2 mg/Kg, 30 min and 6 hours after injury) in a mice model of SCI. The spinal cord trauma was induced by the application of vascular clips to the dura via a four-level T5-T8 laminectomy.Results: Spinal cord injury in mice resulted in severe trauma characterized by edema, neutrophil infiltration and apoptosis. In this study we clearly demonstrated that administration of MK801 attenuated all inflammatory parameters. In fact 24 hours after injury, the degree of spinal cord inflammation and tissue injury (evaluated as histological score), infiltration of neutrophils, NF-κB activation, iNOS, cytokines levels (TNF-α and IL-1β), neurotrophin expression were markedly reduced by MK801 treatment. Moreover, in a separate set of experiments, we have demonstrated that MK801 treatment significantly improved the recovery of locomotory function.Conclusions: Blockade of NMDA by MK801 lends support to the potential importance of NMDA antagonists as therapeutic agents in the treatment of acute spinal cord injury.

Original languageEnglish
Article number31
JournalBMC Neuroscience
Volume12
DOIs
Publication statusPublished - Apr 14 2011

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)

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